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首页> 外文期刊>Physiological and Molecular Plant Pathology >Immunocytochemical localization of beta-1,3-glucanase and chitinase in Fusarium culmorum-infected wheat spikes
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Immunocytochemical localization of beta-1,3-glucanase and chitinase in Fusarium culmorum-infected wheat spikes

机译：镰刀菌感染小麦穗中β-1,3-葡聚糖酶和几丁质酶的免疫细胞化学定位

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摘要

Two antisera raised against acidic beta-1,3-glucanase and acidic chitinase from tobacco were used to investigate the subcellular localization of the two enzymes in Frsarium culmorum-infected wheat spike by means of the immunogold labelling technique. The studies demonstrated that the distribution of beta-1,3-glucanase and chitinase were very similar in the unioculated healthy and infected wheat spikes. The enzymes were localixed mainly in the cell walls of different tissues including the lemma, ovary and rachis of the wheat spoke, while the cytoplasm and organelles of cells in these tissues showed almost no labelling. However, the accumulation of beta-1,3-glucanase and chitinase in the infected wheat spikes differed distinctly between resistant and susceptible wheat cultivars. The labelling densities for the tow enzymes in the ingected lemma, ovary and rachis of the suscceptible cultivar Agent increased only slightly as compared to the corredponding uninoculated healthy tissues, whereas higher laelling densitied of beta-1,3-glucanase and chitinase were found in the infected tissues of wheat spikes from the resistant cultivar Arina compared to the corresponding uninoculated healthy tissues. Furthermore, the labelling of beta-1,3-glucanase and chitinase also occurred over the cell walls of the hyphae in the infected whwat spike, but not over the hyphal cytoplasm. In additon, labelling for the two enzymes was often detected over the cell in infected tissues of the resistant wheat cultivar. The findings reported in the present study indicate that beta-1,3-glucanase and chitinase accumulation in the F. culmorum-infected wheat spike may be involved in resistance to pathogen spread in the host tissue.
机译：使用两种针对烟草中酸性β-1,3-葡聚糖酶和酸性几丁质酶的抗血清，通过免疫金标记技术研究了在Frsarium culmorum感染的小麦穗中两种酶的亚细胞定位。研究表明，在未接种的健康和感染小麦穗中，β-1,3-葡聚糖酶和几丁质酶的分布非常相似。酶主要定位在小麦组织的内膜，子房和叶片的不同组织的细胞壁上，而这些组织中的细胞质和细胞器几乎没有标记。但是，在受感染的小麦穗中，β-1,3-葡聚糖酶和几丁质酶的积累在抗性和易感小麦品种之间明显不同。与未接种的健康组织相比，易感品种Am的外胚层，卵巢和叶片中的丝束酶的标记密度仅略有增加，而在β-1,3-葡聚糖酶和几丁质酶中发现较高的分层密度。与相应的未接种健康组织相比，来自抗性品种Arina的小麦穗感染的组织。此外，β-1，3-葡聚糖酶和几丁质酶的标记也发生在感染的白色穗状花序的菌丝细胞壁上，而不是在菌丝细胞质上。另外，经常在抗性小麦品种的感染组织中的细胞上检测到两种酶的标记。本研究报告的发现表明，在F. culmorum感染的小麦穗中，β-1,3-葡聚糖酶和几丁质酶的积累可能与对病原体在宿主组织中传播的抵抗力有关。

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