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Dehydrated pork manure by-product: effect of a chitosan amendment on bacterial community and common scab incidence.

机译:脱水猪粪副产物:壳聚糖改良剂对细菌群落和常见结ab发病率的影响。

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摘要

Chitosan amendment modified the composition of a microbial community associated with dehydrated pork manure by-product. The amended product (biosolid PC) contained a lower number of anaerobic bacteria than the non-amended product (biosolid P). Chitosan also significantly reduced the fungal population. A 16S rRNA gene bank constructed from DNA extracted from the bacterial community associated with both P and PC biosolids revealed that bacterial orders Xanthomonodales, Pseudomonadales, Enterobacteriales, Burkholderiales, Actinomycetales, Bacillales, Clostridiales and Lactobacillales were found in both biosolids. Bacteria from the Stenotrophomonas genus were abundant in both biosolids. However, the addition of chitosan appeared to induce changes in the population of some bacterial genera. For example, clones carrying a 16S rRNA gene corresponding to the Bacillus genus were doubled in biosolid PC. In field trials carried out to test their effect on common scab incidence, biosolids P and PC were applied as potato seed treatment. Biosolid P increased disease incidence by a factor of 1.33 and 2.85 in two independent experiments. However, when chitosan was added to the seed treatment, the stimulating effect of biosolid P on common scab was cancelled out.
机译:壳聚糖修正案修改了与脱水猪粪副产物有关的微生物群落的组成。修改后的产品(生物固体PC)所含厌氧菌的数量要少于未修改产品(生物固体P)。壳聚糖还显着减少了真菌种群。从与P和PC生物固体相关的细菌群落中提取的DNA构建的16S rRNA基因库显示,细菌纲 Xanthomonodales , Pseudomonadales , Enterobacteriales , Burkholderiales ,放线菌病,杆菌,小球藻和乳酸杆菌 。嗜盐单胞菌属的细菌在这两种生物固体中都丰富。然而,壳聚糖的添加似乎诱导了某些细菌属种群的变化。例如,将带有与芽孢杆菌属相对应的16S rRNA基因的克隆在生物固体PC中加倍。在为测试其对常见their疮发病率的影响而进行的田间试验中,生物固体P和PC被用作马铃薯种子处理剂。在两个独立的实验中,生物固体P使疾病的发病率增加了1.33和2.85倍。但是,在种子处理中加入壳聚糖后,生物固体磷对普通sc的刺激作用被抵消了。

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