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Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions.

机译:腐霉菌的致病性导致可控条件下大豆的种子腐烂和衰减。

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Pythium species cause seed rot (SR) and damping-off (DO) in soybean worldwide. In a previous study, a number of Pythium species were isolated from infected soybean plants across Ontario and Quebec, but their comparative pathogenicities to soybean were not examined. In the present research, 24 isolates from eight Pythium spp. were evaluated for their pathogenicity in causing soybean SR and DO in a greenhouse environment. The effect of temperature on the ability of these isolates to cause SR was also studied. There were significant differences among the eight Pythium spp. for both SR and DO. When tested at 25 degrees C, Pythium ultimum was the most pathogenic species, causing 97.0% SR and 46.4% DO, on average, in the two soybean cultivars used. Pythium aphanidermatum was the second most pathogenic species, resulting in 88.5% SR and 41.8% DO. The two species resulted in significantly greater SR and DO than the other six species tested and were considered highly pathogenic. Of the two cultivars used in these trials, 'Beechwood' was significantly more susceptible than 'Nattawa' to both SR and DO. Temperature had a significant influence on SR caused by Pythium spp. At all four temperatures tested (4 degrees C, 12 degrees C, 20 degrees C and 28 degrees C), P. ultimum was highly pathogenic, while P. arrenomanes, P. coloratum and P. dissotocum were the least pathogenic. The interactions between temperature and Pythium spp. were more pronounced for P. aphanidermatum, which showed an increased percentage of SR with an increase in temperature, and for P. irregulare, P. macrosporum and P. sylvaticum, which showed a decreased percentage of SR with an increase in temperature.
机译:腐霉属物种导致全世界大豆的种子腐烂(SR)和衰减(DO)。在先前的研究中,从安大略省和魁北克省的受感染大豆植物中分离出了许多腐霉属物种,但未检查它们对大豆的比较致病性。在本研究中,从8个腐霉菌中分离出24种。评估了它们在温室环境中引起大豆SR和DO的致病性。还研究了温度对这些分离物引起SR的能力的影响。八个腐霉菌之间有显着差异。对于SR和DO。在25摄氏度下进行测试时,终腐霉菌是最具致病性的物种,在使用的两个大豆品种中,平均导致97.0%的SR和46.4%的DO。 Aphanidermatum 是第二大致病性物种,产生了88.5%的SR和41.8%的DO。与其他六个物种相比,这两个物种导致的SR和DO明显更高,被认为是高致病性的。在这些试验中使用的两个品种中,“山毛榉木”比“纳塔瓦”对SR和DO的敏感性要高得多。温度对由腐霉菌引起的SR有重要影响。在所有四个测试温度下(4摄氏度,12摄氏度,20摄氏度和28摄氏度),P。最终是高致病性的,而 P。 Arrenomanes , P。 coloratum 和 P。 Dissotocum 的致病性最低。温度与腐霉菌 spp之间的相互作用。对于 P更为明显。 aphanidermatum ,其中 P的SR百分比随温度的升高而增加。不规则, P。大孢子菌和 P。 sylvaticum ,随着温度的升高,SR的百分比降低。

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