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DNA methylation biomarkers of cancer: moving toward clinical application.

机译:癌症的DNA甲基化生物标志物:走向临床应用。

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While different markers for cancer diagnosis have been known for at least a decade, the systematic search for biomarkers emerged only several years ago. In this article, I will concentrate on DNA methylation as a dynamic and robust platform for the development of cancer-specific biomarkers. Simultaneous analysis of a growing number of independent methylation events can create increasingly more precise and individualized diagnostics. The differential detection of methylated and unmethylated DNA can be accomplished through either chemical modification or digestion with methylation-sensitive restriction enzyme(s). The benefits and potential pitfalls of both these approaches for clinical sample analysis will be addressed.
机译:尽管至少十年前就已经知道了用于癌症诊断的不同标记,但是对生物标记的系统性搜索仅在几年前才出现。在本文中,我将专注于DNA甲基化作为开发癌症特异性生物标记物的动态而强大的平台。越来越多的独立甲基化事件的同时分析可以创建越来越精确和个性化的诊断。甲基化和未甲基化的DNA的差异检测可以通过化学修饰或用甲基化敏感的限制酶消化来完成。将探讨这两种方法用于临床样本分析的好处和潜在的陷阱。

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