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Mapping of quantitative trait loci conferring resistance to brown spot in flue-cured tobacco (Nicotiana tabacum L.)

机译:定量特征基因座赋予对烤烟褐斑病抗性的图谱(Nicotiana tabacum L.)

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摘要

Brown spot (BS) caused by Alternaria alternata is one of the most destructive foliar diseases affecting tobacco (Nicotiana tabacum L.) production and quality in China. Breeding of BS-resistant cultivars is difficult because the resistance has proved to be quantitatively inherited. To facilitate marker-assisted selection, we carried out a study of mapping quantitative trait loci (QTLs) for BS resistance. We developed an F2 population consisting of 213 individuals from a cross between a BS-susceptible cultivar Changbohuang (CBH) and a BS-resistant cultivar Jinyehuang (JYH) and constructed a genetic map consisting of 196 simple sequence repeat (SSR) markers based on this population. Using disease index (DI) as the indicator of BS resistance, we detected three QTLs located between SSR markers TM20534 and TM10737, TM10589 and TM10216, and TM10443 and PT60669, respectively. The resistant alleles of the three QTLs were all from the resistant parent JYH. The three QTLs together could explain similar to 86% of the DI difference between the two parents in total, with similar to 61% explained by their additive effects. Therefore, the three QTLs will be useful for BS-resistance breeding.
机译:链格孢菌引起的褐斑(BS)是影响中国烟草(Nicotiana tabacum L.)生产和质量的最具破坏性的叶面疾病之一。 BS抗性品种的育种是困难的,因为已证明抗性是定量遗传的。为促进标记辅助选择,我们进行了针对BS抗性的定量性状基因座(QTL)定位研究。我们开发了由BS易感品种Changbohuang(CBH)和BS抗性品种Jinyehuang(JYH)之间的杂交组成的213个个体组成的F2种群,并基于此构建了由196个简单序列重复(SSR)标记组成的遗传图谱人口。使用疾病指数(DI)作为BS抗性的指标,我们检测到位于SSR标记TM20534和TM10737,TM10589和TM10216以及TM10443和PT60669之间的三个QTL。这三个QTL的抗性等位基因均来自抗性亲本JYH。这三个QTL总共可以解释两个亲本之间DI差异的86%,通过相加作用可以解释61%。因此,这三个QTL将对BS抗性育种有用。

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