...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant and cell physiology >A comprehensive expression analysis of the Arabidopsis MICRORNA165/6 gene family during embryogenesis reveals a conserved role in meristem specification and a non-cell-autonomous function. (Special Focus Issue: Plant meristems and organogenesis.)
【24h】

A comprehensive expression analysis of the Arabidopsis MICRORNA165/6 gene family during embryogenesis reveals a conserved role in meristem specification and a non-cell-autonomous function. (Special Focus Issue: Plant meristems and organogenesis.)

机译：拟南芥MICRORNA165 / 6基因家族在胚胎发生过程中的全面表达分析揭示了在分生组织规范中的保守作用和非细胞自主功能。 （特别焦点问题：植物分生组织和器官发生。）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

One of the most fundamental events in plant ontogeny is the specification of the shoot and root apical meristem (SAM and RAM) in embryogenesis. In Arabidopsis, the restricted expression of class III homeodomain leucine zipper (HD-ZIP III) transcription factors (TFs) at the central-apical domain of early embryos is required for the correct specification of the SAM and RAM. Because the expression of HD-ZIP III TFs is suppressed by microRNA165/166 (miR165/6), elucidation of the sites of miR165/6 production and their activity range is a key to understanding the molecular basis of SAM and RAM specification in embryogenesis. Here, we present a comprehensive reporter analysis of all nine Arabidopsis MICRORNA165/166 (MIR165/6) genes during embryogenesis. We show that five MIR165/6 genes are transcribed in a largely conserved pattern in embryos, with their expression being preferentially focused at the basal-peripheral region of embryos. Our analysis also indicated that MIR165/6 transcription does not depend on SCARECROW (SCR) function in early embryos, in contrast to its requirement in post-embryonic roots. Furthermore, by observing the expression pattern of the miR-resistant PHBmu-GFP (green fluorescent protein) reporter, in either the presence or absence of the MIR165Amu transgene, which targets PHBmu-GFP, we obtained data that indicate a non-cell-autonomous function for miR165 in early embryos. These results suggest that miR165, and possibly miR166 as well, has the capacity to act as a positional cue from the basal-peripheral region of early embryos, and remotely controls SAM and RAM specification with their non-cell-autonomous function.
机译：植物个体发育中最基本的事件之一是胚胎发生中芽和根顶端分生组织（SAM和RAM）的规格。在拟南芥中，对于SAM和RAM的正确规格要求，III类同源域亮氨酸拉链（HD-ZIP III）转录因子（TFs）在早期胚胎的中央顶端域中的限制性表达。由于microRNA165 / 166（miR165 / 6）抑制了HD-ZIP III TF的表达，因此阐明miR165 / 6产生的位点及其活性范围是了解胚胎发生中SAM和RAM规格的分子基础的关键。在这里，我们提出了胚胎发育过程中所有九个拟南芥MICRORNA165 / 166（MIR165 / 6）基因的综合报道者分析。我们显示五个MIR165 / 6基因在很大程度上保守的模式在胚胎中转录，其表达优先集中在胚胎的基底-外周区域。我们的分析还表明，与其在胚后根中的需求相反，MIR165 / 6转录不依赖于早期胚胎中的SCARECROW（SCR）功能。此外，通过观察miR耐药的PHBmu-GFP（绿色荧光蛋白）报告基因的表达模式，在是否存在靶向PHBmu-GFP的MIR165Amu转基因的情况下，我们获得了表明非细胞自主性的数据miR165在早期胚胎中的功能。这些结果表明，miR165以及可能的miR166具有从早期胚胎的基底周围区域起位置提示的功能，并通过其非细胞自主功能远程控制SAM和RAM规范。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号