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首页> 外文期刊>Plant and Soil >Isolation of ACC deaminase-producing habitat-adapted symbiotic bacteria associated with halophyte Limonium sinense (Girard) Kuntze and evaluating their plant growth-promoting activity under salt stress.
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Isolation of ACC deaminase-producing habitat-adapted symbiotic bacteria associated with halophyte Limonium sinense (Girard) Kuntze and evaluating their plant growth-promoting activity under salt stress.

机译：分离出与盐生植物柠檬酸柠檬（Girard）Kuntze相关的产生ACC脱氨酶的生境适应共生细菌，并评估其在盐胁迫下的植物生长促进活性。

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Background and aims: Many plant growth-promoting endophytes (PGPE) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity can reduce the level of stress ethylene and assist their host plants cope with various biotic and abiotic stresses. However, information about the endophytic bacteria colonizing in the coastal halophytes is still very scarce. This study aims at isolating efficient ACC deaminase-producing plant growth-promoting (PGP) bacterial strains from the inner tissues of a traditional Chinese folk medicine Limonium sinense (Girard) Kuntze, a halophyte which has high economic and medicinal values grown in the coastal saline soils. Their PGP activity and effects on host seed germination and seedling growth under salinity stress were also evaluated. Methods: A total of 126 isolates were obtained from the surface sterilized roots, stems and leaves of L. sinense (Girard) Kuntze. They were initially selected for their ability to produce ACC deaminase as well as other PGP properties such as production of indole-3-acetic acid (IAA), N2-fixation, and phosphate-solubilizing activities and subsequently identified by the 16S rRNA gene sequencing. For selected strains, seed germination, seedling growth, and flavonoids production in axenically growth L. sinense (Girard) Kuntze seedlings at different NaCl concentrations (0-500 mM) were quantified. Results: Thirteen isolates possessing ACC deaminase activity were obtained. The 16S rRNA gene sequencing analysis showed them to belong to eight genera: Bacillus, Pseudomonas, Klebsiella, Serratia, Arthrobacter, Streptomyces, Isoptericola, and Microbacterium. Inoculation with four of the selected ACC deaminase-producing strains not only stimulated the growth of the host plant but also influenced the flavonoids accumulation. All four strains could colonize and can be re-isolated from the host plant interior tissues. Conclusions: These results demonstrate that ACC deaminase-producing habitat-adapted symbiotic bacteria isolated from halophyte could enhance plant growth under saline stress conditions and the PGPE strains could be appropriate as bioinoculants to enhance soil fertility and protect the plants against salt stress.
机译：背景与目的：许多具有1-氨基环丙烷-1-羧酸酯（ACC）脱氨酶活性的植物促生内生菌（PGPE）可以降低胁迫乙烯的水平，并帮助其宿主植物应对各种生物和非生物胁迫。但是，关于内生细菌在沿海盐生植物中定殖的信息仍然非常匮乏。这项研究的目的是从传统的中药Limonium sinense（Girard）Kuntze的内部组织中分离出有效的产生ACC脱氨酶的植物生长促进（PGP）细菌菌株，该盐生植物在沿海盐水中具有很高的经济和药用价值土壤。还评估了它们在盐分胁迫下的PGP活性及其对寄主种子萌发和幼苗生长的影响。方法：从表面消毒过的L. sinense（Girard）Kuntze的根，茎和叶中分离出126种分离株。最初选择它们的原因是它们具有产生ACC脱氨酶的能力以及其他PGP特性，例如吲哚-3-乙酸（IAA）的生产，N 2 固定和磷酸盐增溶活性，随后通过16S rRNA基因测序鉴定。对于选定的菌株，对在不同NaCl浓度（0-500 mM）下的无性系L. sinense（Girard）Kuntze幼苗中的种子发芽，幼苗生长和类黄酮产量进行了定量。结果：获得13株具有ACC脱氨酶活性的菌株。 16S rRNA基因测序分析显示它们属于八个属：芽孢杆菌，假单胞菌，克雷伯菌，沙雷氏菌，节杆菌，链霉菌，等翅目和微细菌。接种四种选定的产生ACC脱氨酶的菌株，不仅刺激了宿主植物的生长，而且还影响了类黄酮的积累。所有四个菌株都可以定植，并且可以从宿主植物内部组织中重新分离出来。结论：这些结果表明，从盐生植物中分离出能够产生ACC脱氨酶的生境适应共生细菌可以在盐胁迫条件下促进植物生长，而PGPE菌株可以作为生物菌剂来增强土壤肥力并保护植物免受盐胁迫。

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