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Root associated iron oxidizing bacteria increase phosphate nutrition and

机译:根相关的铁氧化细菌增加了磷酸盐的营养,

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Typha angustifolia is a heavy metal tolerant plant that grows in a uranium mine tailings highly contaminated with iron. In this study three iron oxidizing microbes (FeOBs) isolated from Typha rhizoplane were investigated for their role in plant growth promotion (PGP). Their effect on iron nutrition in Typha under iron replete and excess condition was also evaluated. The PGP activities of the FeOBs were studied by measuring their influence on plant growth. To investigate the mechanism of growth promotion their ability to solubilize phosphate, and to produce Indole acetic acid and siderophores were studied. The influence of the FeOBs on root to shoot partitioning of iron was tested by measuring total iron content in roots and shoots treated with microbes. The FeOBs were named as Paenibacillus cookii JGR8, (MTCC12002), Pseudomonas jaduguda JGR2 (LMG25820) and Bacillus megaterium JGR9 (MTCC12001). The siderophore producers, influenced iron accumulation in the plant root. Additionally P. pseudoalcaligenes JGR2 increased shoot iron content overcoming the root- shoot barrier that allows Typha to exclude metals from its shoot. Among the PGP mechanisms tested, ability to solubilize phosphate appeared to be most significant for increasing the plant biomass. FeOBs that produce siderophore increased iron content in plant and therefore can be of immense biotechnological importance. However Biomass increase was directly correlated with increased phosphate acquisition and not with enhanced iron accumulation in Typha
机译:香蒲(Typha angustifolia)是一种重金属耐受植物,生长在被铁高度污染的铀矿尾矿中。在这项研究中,研究了从香蒲根际飞机中分离出的三种铁氧化微生物(FeOB)在植物生长促进(PGP)中的作用。还评估了它们在铁过量和过量条件下对香蒲中铁营养的影响。通过测量FeOB对植物生长的影响来研究其PGP活性。为了研究促进生长的机理,研究了它们溶解磷酸盐的能力,以及生产吲哚乙酸和铁载体的能力。通过测量微生物处理过的根和芽中的总铁含量,测试了FeOB对根对铁的芽分配的影响。 FeOBs被命名为Paenibacillus cookii JGR8(MTCC12002),假单胞菌Jaduguda JGR2(LMG25820)和巨大芽孢杆菌JGR9(MTCC12001)。铁载体的生产者影响了植物根系中铁的积累。此外,假拟疟原虫JGR2增加了芽中铁的含量,从而克服了根茎屏障,使香蒲能够从芽中排除金属。在测试的PGP机制中,溶解磷酸盐的能力似乎对于增加植物生物量最为重要。产生铁载体的FeOB增加了植物中的铁含量,因此可能具有巨大的生物技术重要性。然而,生物量的增加与磷获取量的增加直接相关,而不与香蒲中铁的积累增加相关。

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