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The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists

机译:使用不含氮的半固体培养基从非豆科植物中分离固氮细菌的技术:微生物学家实用指南

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摘要

Nitrogen-fixing bacteria or diazotrophs have been isolated for many years using different formulations of N-free semi-solid media. However, the strategies used to isolate them, and the recipes of these media, are scattered through the published literature and in other sources that are more difficult to access and which are not always retrievable. Therefore, the aim of this work was to collate the various methods and recipes, and to provide a comprehensive methodological guide and their use by the scientific community working in the field of biological nitrogen fixation (BNF), particularly with non-leguminous plants.
机译:使用不同形式的无氮半固体培养基分离固氮细菌或重氮菌已有多年历史。但是,用于隔离它们的策略以及这些介质的配方散布在已出版的文献中,以及散布在更难以获取且并不总是可检索的其他来源中。因此,这项工作的目的是整理各种方法和配方,并为从事生物固氮(BNF)领域的科学界(特别是非豆科植物)提供全面的方法学指导和使用。

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