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首页> 外文期刊>Pigment cell research >Isolation of the Atlantic salmon tyrosinase gene family reveals heterogenous transcripts in a leukocyte cell line
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Isolation of the Atlantic salmon tyrosinase gene family reveals heterogenous transcripts in a leukocyte cell line

机译：大西洋鲑鱼酪氨酸酶基因家族的分离揭示白细胞系中的异源转录本

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In ectothermic vertebrates, visceral organs harbor melanin-containing cells. Their ability as pigment producers is nevertheless disputed. To address expression of the key genes for melanogenesis in Atlantic salmon (Salmo salar), a tyrosinase-positive leukocyte cell line (SHK-1) and skin were used to obtain full-length tyrosinase (Tyr), tyrosinase-like protein-1 (Tyrp1), and dopachrome tautomerase (Dct) mRNA transcripts. In the SHK-1 cells, two different Tyrp1 transcripts were identified, one lacking exon 1. However, only the full-length version of Tyrp1 was identified in the skin. Sequencing of Tyrp1 genomic region revealed that the two Tyrp1 transcripts might originate from two different loci, possibly a result of pseudo-tetraploidity of the Atlantic salmon genome. Expression of Tyr, Tyrp1 and Dct was investigated by quantitative real-time reverse transcriptase polymerase cain reaction showing highest expression in the SHK-1 cell line and skin, intermediate in pronephros, and negligible or absent in liver and muscle. Histological approaches were used to demonstrate melanin and revealed presence of melanized cells in skin, kidney and liver, and absence of such cells in muscle. In addition to verify melanin synthesis abilities of visceral-located cells, our results indicate loci-specific transcription differences between populations of melanin-producing cells in Atlantic salmon.
机译：在外热脊椎动物中，内脏器官含有含黑色素的细胞。然而，他们作为颜料生产商的能力存在争议。为了解决大西洋鲑（Salmo salar）黑色素生成关键基因的表达问题，使用了酪氨酸酶阳性白细胞细胞系（SHK-1）和皮肤来获得全长酪氨酸酶（Tyr），酪氨酸酶样蛋白1（ Tyrp1）和多巴色素互变异构酶（Dct）mRNA转录本。在SHK-1细胞中，鉴定出两种不同的Tyrp1转录本，其中一个缺少外显子1。但是，在皮肤中仅鉴定到了全长的Tyrp1。 Tyrp1基因组区域的测序表明，两个Tyrp1转录本可能源自两个不同的基因座，这可能是大西洋鲑鱼基因组伪四倍体的结果。通过定量实时逆转录酶聚合酶cain反应研究了Tyr，Tyrp1和Dct的表达，该反应在SHK-1细胞系和皮肤中表达最高，在前肾中居中间，在肝脏和肌肉中可忽略或不存在。组织学方法用于证明黑色素，并揭示皮肤，肾脏和肝脏中黑色素细胞的存在，而肌肉中不存在此类细胞。除了验证位于内脏的细胞的黑色素合成能力外，我们的结果还表明大西洋鲑鱼黑色素产生细胞群之间的基因座特异性转录差异。

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