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首页> 外文期刊>Pigment cell research >Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin
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Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin

机译：培养的人黑色素细胞中色素沉着的多样性是由于黑色素类型和数量的差异

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Cultured human melanocytes differ tremendously in visual pigmentation, and recapitulate the pigmentary phenotype of the donor's skin. This diversity arises from variation in type as well as quantity of melanin produced. Here, we measured contents of eumelanin (EM) and pheomelanin (PM) in 60 primary human melanocyte cultures (51 neonatal and nine adults), and correlated some of these values with the respective activity and protein levels of tyrosinase, and the melanocortin-1 receptor (MC1R) genotype. Melanocytes were classified into four phenotypes (L, L+, D, D+) as depicted by visual pigmentation using light microscopy, and by the pigmentary phenotype of the donor's skin. There were large differences in total melanin (TM) and EM, which increased progressively for L, L+, D and D+ melanocytes. TM content, the sum of EM and PM, showed a good correlation with TM measured spectrophotometrically, and with the activity and protein levels of tyrosinase. Log EM/PM ratio did not correlate with MC1R genotype. We conclude that: (i) EM consistently correlates with the visual phenotype; (ii) lighter melanocytes tend to be more pheomelanic in composition than darker melanocytes; (iii) in adult melanocyte cultures, EM correlates with the ethnic background of the donors (African-American > Indian > Caucasian); and (iv) MC1R loss-of-function mutations do not necessarily alter the phenotype of cultured melanocytes.
机译：培养的人黑素细胞在视觉色素沉着方面有很大差异，并概括了供体皮肤的色素表型。这种多样性源于黑色素类型和数量的变化。在这里，我们测量了60种主要人类黑素细胞培养物中（51个新生儿和9个成年人）的Eumelanin（EM）和pheomelanin（PM）的含量，并将其中一些值与酪氨酸酶和melanocortin-1的各自活性和蛋白质水平相关联受体（MC1R）基因型。如通过使用光学显微镜的目视色素沉着和通过供体皮肤的色素表型所描绘的，黑素细胞被分为四种表型（L，L +，D，D +）。总黑色素（TM）和EM有很大差异，对于L，L +，D和D +黑色素细胞，这些差异逐渐增加。 TM含量，EM和PM的总和，与分光光度法测得的TM以及酪氨酸酶的活性和蛋白质水平具有良好的相关性。 Log EM / PM比值与MC1R基因型无关。我们得出以下结论：（i）EM与视觉表型始终相关; （ii）较黑的黑素细胞比较黑的黑素细胞在成分上更容易冒泡。 （iii）在成人黑素细胞培养物中，EM与供体的种族背景相关（非裔美国人>印度人>高加索人）; （iv）MC1R功能丧失突变不一定改变培养的黑素细胞的表型。

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