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首页> 外文期刊>Pigment cell research >Hemocyanin in the exoskeleton of crustaceans: enzymatic properties and immunolocalization
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Hemocyanin in the exoskeleton of crustaceans: enzymatic properties and immunolocalization

机译：甲壳类外骨骼中的血蓝蛋白：酶学性质和免疫定位

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摘要

We investigated the enzymatic properties and immunohistochemical localization of cuticular hemocyanin, a known oxygen transporter in the prawn Penaeus japonicus. The molecular weight of hemocyanin purified from the cuticle was estimated to be 67-77 k using SDS-PAGE, and the purified protein was effectively converted into a phenoloxidase-like enzyme by an SDS-treatment. The activated enzyme catalyzed the o-hydroxylation of monophenols and the oxidation of o-diphenols and was inhibited by typical inhibitors of phenoloxidase. These characteristics were nearly identical to the enzymatic properties of hemolymph hemocyanin. Immunological detection showed a diffuse distribution of hemocyanin over the exocuticle and endocuticle, and a higher signal level was observed in the latter. Based on these results, roles of hemocyanin in various physiological processes such as immune response and sclerotization of the cuticle were discussed.
机译：我们调查了对虾血红蛋白的酶学性质和免疫组化定位，一种已知的虾对虾对氧转运蛋白。使用SDS-PAGE，从表皮纯化的血蓝蛋白的分子量估计为67-77k，并且通过SDS处理将纯化的蛋白质有效地转化为酚氧化酶样酶。活化的酶催化单酚的邻羟基化和邻二酚的氧化，并被典型的酚氧化酶抑制剂抑制。这些特征与血淋巴血蓝蛋白的酶学性质几乎相同。免疫学检测显示，血蓝蛋白在表皮和内皮层上分散分布，并且在后者中观察到较高的信号水平。基于这些结果，讨论了血蓝蛋白在各种生理过程中的作用，例如免疫反应和表皮的硬化。

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