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首页> 外文期刊>Pigment cell research >Widespread expression of the bovine Agouti gene results from at least three alternative promoters
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Widespread expression of the bovine Agouti gene results from at least three alternative promoters

机译：牛Agouti基因的广泛表达是由至少三个其他启动子导致的

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In wild-type mice, it is well known that Agouti is only expressed in skin where it controls the banded-hair phenotype. As a first step to investigate the physiological role of Agouti in cattle, we isolated the corresponding gene and studied its expression pattern. We found no evidence of coding-region sequence variation within and between eight breeds representing a large panel of coat colour phenotypes. We detected by northern hybridization two Agouti mRNA isoforms in brain, heart, lung, liver, kidney, spleen and a third in skin. We characterized the full-length Agouti transcript in skin and isolated the 5'UTR of two mRNAs expressed in the other tissues. The three mRNAs have the same coding region but differ by their 5' untranslated regions. Upstream regulatory sequences display two alternative promoters involved with the broad expression in tissues other than skin. Interestingly, these sequences are highly homologous to upstream sequences of the orthologous human (76-85% identity) and pig (82-86% identity) ASIP genes. In addition to its potential role in pigmentation (as seen in mice), we suggest that bovine Agouti could be involved in various physiological functions. Furthermore, the significant homology between cattle, pig and human regulatory sequences indicate that these orthologous genes are regulated alike. Lastly, since the 5'UTR of many eukaryotic mRNAs are physiologically relevant, their impact on bovine Agouti mRNA performance is discussed.
机译：在野生型小鼠中，众所周知Agouti仅在控制条带状表型的皮肤中表达。作为研究牛膝肉在牛中的生理作用的第一步，我们分离了相应的基因并研究了其表达模式。我们发现没有证据表明代表一个大的外套颜色表型的八个品种之内和之间的编码区序列变异。通过北部杂交，我们在脑，心脏，肺，肝，肾，脾脏中发现了两个Agouti mRNA亚型，在皮肤中发现了三分之一。我们表征了皮肤中的全长Agouti转录本，并分离了在其他组织中表达的两个mRNA的5'UTR。这三种mRNA具有相同的编码区，但其5'非翻译区有所不同。上游调节序列显示了两个替代启动子，涉及在皮肤以外的组织中的广泛表达。有趣的是，这些序列与直系同源人（76-85％相同性）和猪（82-86％相同性）ASIP基因的上游序列高度同源。除了其在色素沉着中的潜在作用（如在小鼠中观察到的）外，我们建议牛长吻鳄可能参与多种生理功能。此外，牛，猪和人类调控序列之间的显着同源性表明，这些直系同源基因也受到同样的调控。最后，由于许多真核mRNA的5'UTR具有生理相关性，因此讨论了它们对牛Agouti mRNA性能的影响。

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