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首页> 外文期刊>Pigment cell research >Co-localization of β1,6-Branched Oligosaccharides and Coarse Melanin in Macrophage-Melanoma Fusion Hybrids and Human Melanoma Cells in vitro
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Co-localization of β1,6-Branched Oligosaccharides and Coarse Melanin in Macrophage-Melanoma Fusion Hybrids and Human Melanoma Cells in vitro

机译：β1，6-分支寡糖和粗黑素在巨噬细胞-黑素瘤融合杂种和人黑素瘤细胞中的共定位

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摘要

Fusion hybrids between normal macrophages and Cloudman S91 melanoma cells were shown earlier to have increased metastatic potential, along with high expression of β1,6-N-acetylglucosaminyltransferase V (GnT-V) and β1,6-branched oligosaccharides. Curiously, hybrids, but not parental melanoma cells, also produced oarse melanin'-autophagic vesicles with multiple melanosomes. Since b1,6-branched oligosaccharides were known to be associated with metastasis, and coarse melanin had been described in invasive human melanomas, we looked for potential relationships between the two. Using lectin/immuno-histochemistry, we analyzed cell lines producing coarse melanin for β1,6-branched oligosaccharides; gp100/pmel-17 (a melanosomal structural component) and CD63 (a late endosome/lysosome component associated with melanoma and certain other human cancers). Cell lines used in this study were hybrid 94-H48, a highly metastatic, macrophage-melanoma experimental fusion hybrid; 6neo mouse melanoma cells, the weakly metastatic, parental fusion partner; and SKMel-23, a human melanoma cell line derived from a metastasis. Coarse melanin granules were prominent both in hybrids and in SKmel-23 cells, and co-localized with stains for β1,6-branched oligosaccharides, gp100/pmel 17, and CD63. This is the first report of this phenotype being expressed in vitro, although co-expression of β1,6-branched oligosaccharides and coarse melanin was recently shown to be a common and pervasive characteristic in archival specimens of human melanomas, and was most prominent in metastases. The results suggest that pathways of melanogenesis in melanoma may differ significantly from those in normal melanocytes. In vitro expression of this phenotype provides new biological systems for more detailed analyses of its genesis and regulation at the molecular genetic level.
机译：正常巨噬细胞和Cloudman S91黑色素瘤细胞之间的融合杂种已被证明具有更高的转移潜力，并具有高表达的β1,6-N-乙酰氨基葡萄糖氨基转移酶V（GnT-V）和β1,6-支链寡糖。奇怪的是，杂种而不是亲代黑素瘤细胞也产生了带有多个黑素体的黑色素'自噬囊泡。由于已知b1,6-支链寡糖与转移有关，并且已在侵袭性人黑素瘤中描述了粗黑素，因此我们寻找两者之间的潜在关系。使用凝集素/免疫组织化学方法，我们分析了产生粗黑色素的β1,6-支化寡糖细胞系。 gp100 / pmel-17（一种黑素体结构成分）和CD63（一种与黑色素瘤和某些其他人类癌症相关的晚期内体/溶酶体成分）。在这项研究中使用的细胞系是杂种94-H48，一种高度转移性的巨噬细胞-黑色素瘤实验性融合杂种。 6neo小鼠黑色素瘤细胞，弱转移的父母融合伴侣; SKMel-23，一种源自转移的人黑素瘤细胞系。粗糙的黑色素颗粒在杂种和SKmel-23细胞中均很突出，并与β1,6-支链低聚糖，gp100 / pmel 17和CD63的染色剂共定位。这是该表型在体外表达的第一份报道，尽管最近发现在人黑素瘤的档案标本中共表达β1,6-支链寡糖和粗黑素是普遍和普遍的特征，并且在转移中最为突出。结果表明，黑色素瘤中黑色素生成的途径可能与正常黑色素细胞中的明显不同。该表型的体外表达提供了新的生物学系统，用于在分子遗传学水平上对其发生和调控进行更详细的分析。

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