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A resin buffered method for controlling metal speciation in nutrient solutions for plant toxicity tests

机译：用于植物毒性测试的控制营养液中金属形态的树脂缓冲方法

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Root elongation tests are sensitive bioassays for testing metal toxicity in nutrient solutions. The metal speciation and, hence, metal exposure conditions are little controlled in the traditional set-up. A resin buffered solution system was developed to overcome this issue. Barley (Hordeum vulgare L.) root elongation was tested in aerated 140 mL solution batch systems supplied with 3.3 g Dowex resin for two plants. Copper toxicity was measured in presence or absence of the resin (+R/-R) and in presence or absence of a metal complexing ligand (+NTA; nitrilotriacetic acid/-NTA). In addition, the toxicity in the traditional set without resin and with daily solution replacement was included as a reference. Metal desorption from the resin is fast in these systems (k = 0.82 h(-1)). Total dissolved Cu roughly halved during 4 days in -R/-NTA systems due to uptake, while it increased by 30 % in the +R/-NTA, probably due to complexation reactions by root-derived molecules. The toxicity (50 % reduction in root length, EC50) of the initial free Cu2+ was equal in all resin or chelate buffered systems and in the solutions with daily replacement, whereas this threshold was significantly larger in the -R/-NTA due to Cu2+ uptake and complexation reactions. The resin method is a convenient system for high throughput screening of metal toxicity and avoids uncertainties in metal speciation inherent to chelator buffered systems. Details are given how to prepare the resin to obtain a target metal ion activity.
机译：根伸长测试是用于测试营养液中金属毒性的灵敏生物测定法。在传统装置中，金属形态和因此的金属暴露条件几乎不受控制。为了克服这个问题，开发了树脂缓冲溶液系统。大麦（Hordeum vulgare L.）根系伸长率在充气的140 mL溶液批处理系统中进行测试，该系统配有3.3 g Dowex树脂，用于两株植物。在存在或不存在树脂（+ R / -R）和存在或不存在金属络合配体（+ NTA;次氮基三乙酸/ -NTA）的情况下测量铜的毒性。此外，在常规环境中不使用树脂和每天更换溶液的毒性也作为参考。在这些系统中，金属从树脂中的解吸速度很快（k = 0.82 h（-1））。由于吸收，在-R / -NTA系统中溶解的Cu总量在4天之内大致减少了一半，而在+ R / -NTA中增加了30％，这可能是由于根源分子的络合反应所致。在所有树脂或螯合缓冲系统以及每天更换的溶液中，初始游离Cu2 +的毒性（根长减少50％，EC50）均相等，但是由于Cu2 +，该阈值在-R / -NTA中明显更大摄取和络合反应。树脂法是用于高通量金属毒性筛选的便捷系统，并且避免了螯合剂缓冲系统固有的金属形态不确定性。详细说明了如何制备树脂以获得目标金属离子活性。

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