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首页> 外文期刊>Plant and Soil >Establishment and survival of the South African legume Lessertia spp. and rhizobia in Western Australian agricultural systems
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Establishment and survival of the South African legume Lessertia spp. and rhizobia in Western Australian agricultural systems

机译：南非豆科植物Lessertia spp的建立和生存。西澳大利亚州农业系统中的根瘤菌和根瘤菌

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The South African herbaceous legume species Lessertia capitata, L. diffusa, L. excisa L. incana and L. herbacea were introduced to Australia to assess plant establishment and survival, as well as the saprophytic ability of their root nodule bacteria (RNB). Five Lessertia spp., were inoculated with selected RNB strains and were sown in five different agroclimatic areas of the Western Australian wheat-belt during 2007 and 2008. Plant population and summer survival were evaluated in situ. Soil samples and nodules from host plants were also taken from each site. The re-isolated rhizobia were RPO1-PCR fingerprinted and their partial dnaK and nodA genes were sequenced to confirm their identity. Plants achieved only poor establishment followed by weak summer survival. More than 83 % of the rhizobia re-isolated from Lessertia did not correlate with the original inoculants' fingerprints, and were identified as Rhizobium leguminosarum. The nodA sequences of the naturalised strains were also clustered with R. leguminosarum sequences, thus eliminating the likelihood of lateral gene transference from Mesorhizobium and suggesting a competition problem with indigenous rhizobia. The stressful soil conditions and high numbers of resident R. leguminosarum strains in Western Australian soils, and their ability to rapidly nodulate Lessertia spp. but not fix nitrogen are likely to preclude the adoption of Lessertia as an agricultural legume in this region.
机译：将南非草本豆科植物小叶莴苣种，白花L. diffusa，L。excisa L. incana和L.草本植物引入澳大利亚，以评估植物的建立和存活以及其根瘤细菌（RNB）的腐生能力。在2007年至2008年期间，用选定的RNB菌株接种了5株小白菜菌，并在西澳大利亚州小麦带的5个不同的农业气候地区进行了播种。对植物种群和夏季存活情况进行了现场评估。还从每个地点采集了寄主植物的土壤样品和结核。对重新分离的根瘤菌进行RPO1-PCR指纹图谱分析，并对它们的部分dnaK和nodA基因进行测序，以确认它们的身份。植物仅建立不好的状态，其后的夏季存活率很低。从莱瑟氏菌中重新分离出的根瘤菌中，有超过83％与原始接种物的指纹图谱不相关，被鉴定为豆科根瘤菌。归化菌株的nodA序列也与豆科根瘤菌序列聚在一起，从而消除了从根瘤菌侧向转移基因的可能性，并提出了与本土根瘤菌的竞争问题。在澳大利亚西部土壤中，土壤环境条件恶劣，豆科植物常住的豆科菌菌株数量众多，它们能够快速结瘤小叶菜。但不能固定氮肥可能会阻止该地区将莱瑟氏菌用作农业豆类。

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