A fluorescent antibody assay for hyphae and glomalin from arbuscularmycorrhizal fungi

Wright SF

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A fluorescent antibody assay for hyphae and glomalin from arbuscularmycorrhizal fungi

机译：丛枝菌根真菌中菌丝和gloomalin的荧光抗体测定

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Studies on the role of arbuscular mycorrhizal (AM) fungi in soil have been aided by the use of a monoclonal antibody that detects a molecule common to all isolates of these fungi studied to date. The molecule, glomalin, is a glycoprotein that forms on hyphae, but apparently sloughs off and adheres to soil particles or imbedded plastic mesh. An indirect immunofluorescence (IF) assay is described for detection of glomalin on hyphae attached to roots, in roots, on hyphae traps and on the surface of soil aggregates. Small sieves are used to process hyphae attached to roots and soil aggregates. Glomalin on hyphae and glomalin attached to plastic or nylon are assayed on a 1 cm(2) section of meshes. Examples of IF assay results are shown and discussed.

机译：通过使用单克隆抗体来检测土壤中丛枝菌根（AM）真菌的作用，该单克隆抗体可检测迄今研究的这些真菌的所有分离物共有的分子。胶质球蛋白分子是在菌丝上形成的糖蛋白，但显然会脱落并粘附在土壤颗粒或嵌入的塑料网上。描述了一种间接免疫荧光（IF）检测方法，用于检测附着于根部的菌丝，根部，菌丝捕获器和土壤团聚体表面的胶质球蛋白。小筛子用于处理附着在根和土壤聚集体上的菌丝。菌丝上的glomalin和附着在塑料或尼龙上的gloomalin在网眼的1 cm（2）切片上测定。显示并讨论了IF测定结果的实例。

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《Plant and Soil》 |2000年第2期|共7页
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Wright SF;
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正文语种 eng
中图分类植物生态学和植物地理学;
关键词
Glycoprotein; Immunofluorescence; Monoclonal antibody; Soil biology.; Linked immunosorbent-assay; Monoclonal-antibodies; Soil.; Immunofluorescence; Identification; Colonization; Stability; Antigens.; Protein; Roots;

机译：糖蛋白;免疫荧光;单克隆抗体;土壤生物学;连锁免疫吸附测定;单克隆抗体;土壤;免疫荧光;鉴定;定殖;稳定性;抗原;蛋白质;根;

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1. A fluorescent antibody assay for hyphae and glomalin from arbuscularmycorrhizal fungi [J] . Wright SF Plant and Soil . 2000,第2期

机译：丛枝菌根真菌中菌丝和gloomalin的荧光抗体测定
2. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi [J] . Wright SF, Upadhyaya A Plant and Soil . 1998,第1期

机译：对土壤的聚集稳定性和gloomalin的调查，gloomalin是丛枝菌根真菌的菌丝产生的糖蛋白
3. Cd-induced production of glomalin by arbuscular mycorrhizal fungus (Rhizophagus irregularis) as estimated by monoclonal antibody assay [J] . Malekzadeh Elham, Aliasgharzad Nasser, Majidi Jafar, Environmental Science and Pollution Research . 2016,第20期

机译：单克隆抗体测定法估计丛枝菌根真菌（Rhizophagus Regularis）镉诱导的gloomalin的产生
4. Coastal pollution monitoring by an automatic multisampler coupled with a fluorescent antibody assay [C] . G. Zappala, G. Caruso, E. Crisafi International Conference on Environment Problems in Coastal Regions; 2004; Alicante; ES . 2004

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5. Natural Killer Cell Response During Acute Toxoplasmosis & Development of an Antibody-dependent Phagocytosis Assay for the Assessment of HIV-Specific Antibodies [D] . Fettel, Kevin D. 2019

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A fluorescent antibody assay for hyphae and glomalin from arbuscularmycorrhizal fungi

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