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首页> 外文期刊>Plant and Soil >Rhizobial counts in peat inoculants vary amongst legume inoculant groups at manufacture and with storage: implications for quality standards
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Rhizobial counts in peat inoculants vary amongst legume inoculant groups at manufacture and with storage: implications for quality standards

机译：豆类孕育剂的根瘤菌计数在制造和储存过程中因豆类孕育剂组而异：对质量标准的影响

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Inoculation of legumes at sowing with rhizobia has arguably been one of the most cost-effective practices in modern agriculture. Critical aspects of inoculant quality are rhizobial counts at manufacture/registration and shelf (product) life.In order to re-evaluate the Australian standards for peat-based inoculants, we assessed numbers of rhizobia (rhizobial counts) and presence of contaminants in 1,234 individual packets of peat-based inoculants from 13 different inoculant groups that were either freshly manufactured or had been stored at 4 A degrees C for up to 38 months to determine (a) rates of decline of rhizobial populations, and (b) effects of presence of contaminants on rhizobial populations. We also assessed effects of inoculant age on survival of the rhizobia during and immediately after inoculation of polyethylene beads.Rhizobial populations in the peat inoculants at manufacture and decline rates varied substantially amongst the 13 inoculant groups. The most stable were Sinorhizobium, Bradyrhizobium and Mesorhizobium with Rhizobium, particularly R. leguminosarum bv. trifolii the least stable. The presence of contaminants at the 10(-6) level of dilution, i.e. > log 6.7 g(-1) peat, reduced rhizobial numbers in the stored inoculants by an average of 37 %. Survival on beads following inoculation improved 2-3 fold with increasing age of inoculant.We concluded that the Australian standards for peat-based rhizobial inoculants should be reassessed to account for the large differences amongst the groups in counts at manufacture and survival rates during storage. Key recommendations are to increase expiry counts from log 8.0 to log 8.7 rhizobia g(-1) peat and to have four levels of inoculant shelf life ranging from 12 months to 3 years
机译：豆科植物接种根瘤菌时，可以说是现代农业中最具成本效益的做法之一。孕育剂质量的关键方面是生产/注册和货架（产品）寿命中的根瘤菌计数。为了重新评估澳大利亚基于泥炭的孕育剂标准，我们评估了1,234个人中的根瘤菌数量（根瘤菌数量）和污染物的存在。一包来自13种不同接种物的泥炭基接种物，这些包装物是新鲜制造的或已在4 A摄氏度下保存长达38个月，以确定（a）根瘤菌数量下降的速度，以及（b）存在的根瘤菌的影响根瘤菌种群上的污染物。我们还评估了接种年龄对接种聚乙烯珠粒期间和接种后根瘤菌存活的影响。在13种接种物组中，泥炭接种物的根瘤菌种群在生产和下降速率上有很大差异。最稳定的是中华根瘤菌，缓生根瘤菌和中根瘤菌以及根瘤菌，尤其是豆科植物根瘤菌。 Trifolii最不稳定。稀释度为10（-6）的污泥的存在，即> log 6.7 g（-1）泥炭，使储存的接种物中的根瘤菌数量平均减少了37％。随着接种剂年龄的增加，接种后珠子的存活率提高了2-3倍。我们得出的结论是，应重新评估澳大利亚基于泥炭的根瘤菌接种剂标准，以考虑各组之间在制造和储存期间的计数上的巨大差异。关键建议是将过期的根瘤菌泥炭从8.0日志增加到8.7日志g（-1），并使接种物的保质期达到四个级别，范围从12个月到3年不等

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