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首页> 外文期刊>Plant and Soil >Characterisation and differentiation of indigenous rhizobia isolated from Canarian shrub legumes of agricultural and ecological interest.
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Characterisation and differentiation of indigenous rhizobia isolated from Canarian shrub legumes of agricultural and ecological interest.

机译：从加纳利灌木豆科植物中分离的具有农业和生态意义的土著根瘤菌的特征和分化。

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摘要

In the course of a study on rhizobia nodulating six indigenous legume shrubs from the Canary Islands (Chamaecytisus proliferus subsp. angustifolius and proliferus, Teline canariensis, T. stenopetala, Adenocarpus foliolosus and Spartocytisus supranubius), one Rhizobium and 27 Bradyrhizobium Canarian isolates were characterized. It was found that those ascribed to Bradyrhizobium were promiscuous and formed effective nodules not only on their original host but on C. proliferus subsp. proliferus as well. However, Rhizobium isolate RES-1 was more specific and only nodulated on its host (T. canariensis). The serotyping of these isolates required a broad antisera panel due to the great antigenic diversity of these rhizobia, which appeared to be due to differences in their lipopolysaccharides, the main antigenic determinants, which showed great structural diversity. The 28 isolates studied produced 22 easily distinguishable electrophoretic profiles of lipopolysaccharides. Protein or plasmid electrophoretic profiles were equally or less discriminating than the lipopolysaccharides profiles and were more difficult to compare. The comparison of the lipopolysaccharide electrophoretic patterns is a more reliable and discriminating method than serotyping or electrophoretic protein and plasmid profile analysis for the identification of Bradyrhizobium strains. No correlation between the lipopolysaccharide profiles of the isolates and the plant from which they were obtained or their geographical origin was observed.
机译：在一项根瘤菌研究中，结节来自加那利群岛的6种土著豆类灌木丛（Chamaecytisus proliferus anspifolius和proliferus，Teline canariensis，T.stenopetala，Adenocarpus foliolosus和Spartocytisus supranubius），1个根瘤菌和27个Bradyrhizob。发现归因于缓生根瘤菌的那些是混杂的，并且不仅在其原始宿主上而且在C.proliferus亚种上形成有效的结节。增生。但是，根瘤菌分离株RES-1具有更高的特异性，仅在其寄主（canariensis）上有根瘤。这些分离物的血清分型需要宽泛的抗血清检测，因为这些根瘤菌具有很大的抗原多样性，这似乎是由于它们的脂多糖（主要的抗原决定簇）存在差异，显示了极大的结构多样性。研究的28种分离物产生了22种易于区分的脂多糖电泳图谱。蛋白质或质粒电泳图谱与脂多糖图谱具有相同或更少的区别，并且比较起来比较困难。脂多糖电泳图谱的比较是一种比血清分型或电泳蛋白质和质粒谱分析更可靠和更具判别性的方法，可用于鉴定缓生根瘤菌菌株。在分离物的脂多糖谱与从中获得它们的植物或它们的地理起源之间没有相关性。

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