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首页> 外文期刊>Plant and Soil >Rhizosphere microflora of winter wheat plants cultivated under elevated CO2.
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Rhizosphere microflora of winter wheat plants cultivated under elevated CO2.

机译：在升高的CO2下种植的冬小麦植物的根际微生物区系。

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A study was made of the effect of elevated atmospheric CO2 on rhizosphere microorganisms in a hydroponic system where young wheat plants provided the only source of C for microorganisms. Plants were cultivated in mineral solution in sterile silica sand and exposed to control (ambient) and elevated (double) CO2 concentrations for periods of 13, 20, 25 and 34 days. Microbial biomass C (C content in fractions of 0.3-2.7 鎚) was not affected by the elevated CO2 concentration during the first 25 days ofplant growth and was increased after 34 days of plant growth. The content of poly-beta-hydroxybutyrate (PHB) reserve compounds decreased significantly in the elevated CO2 treatment after 25 and 34 days. It was accompanied by a shift in bacterial distribution towards the nutritional groups utilizing more complex organic material. A coincidence of several events connected with plant and microbial carbon economy was observed in the system under elevated CO2 on the 25th day. A modification of the GC-MS method for the detection of low levels of PHB compounds in natural samples was developed. The lipids fractionation step was excluded and EI MS/MS detection of the main fragment ions of the derivatized compound was used. This guarantees that the ion profileshave a high signal:noise ratio at the correct retention time. The detection limit is then ?0 pg/g of sand or soil. The rhizosphere microflora responded very sensitively to the short-term changes in C partitioning in plants caused by the elevated CO2.
机译：在水培系统中，大气CO2浓度升高对根际微生物的影响进行了研究，在该系统中，年轻的小麦植株为微生物提供了唯一的碳源。将植物在无菌硅砂中的矿物质溶液中培养，然后暴露于对照（环境）和升高的（两倍）CO2浓度下达13、20、25和34天。微生物生物量碳（碳含量为0.3-2.7锤的分数）在植物生长的前25天不受二氧化碳浓度升高的影响，而在植物生长34天后则升高。在经过25天和34天的高CO2处理后，聚β-羟基丁酸酯（PHB）保留化合物的含量显着下降。它伴随着细菌分布向利用更复杂有机材料的营养族转移。在第25天，在二氧化碳浓度升高的情况下，系统中发生了与植物和微生物碳经济相关的若干事件的重合。改进了GC-MS方法的检测方法，以检测天然样品中的低水平PHB化合物。排除了脂质分离步骤，并使用EI MS / MS检测衍生化合物的主要片段离子。这样可以确保离子分布在正确的保留时间处具有较高的信噪比。则检测极限为沙或土壤的≤0pg / g。根际微生物区系对二氧化碳升高引起的植物碳分配的短期变化非常敏感。

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