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首页> 外文期刊>Plant and Soil >Phytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat
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Phytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat

机译:巴西偶氮螺旋藻野生型及其突变菌株在IAA生产中对小麦的光刺激作用

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摘要

Auxin production by Azospirillum is important in the observed plant growth promoting effect. By using different genetically modified strains, the contribution of auxin biosynthesis by A. brasilense in altering root morphology was evaluated in a plate assay. Inoculation with the wild type strains A. brasilense Sp245 and Sp7 resulted in a strong decrease in root length and increase in root hair formation. This effect was abolished when inoculating with an ipdC mutant of A. brasilense. The ipdC gene encodes a key enzyme in the IPyA pathway of IAA synthesis by A. brasilense. On the other hand, the observed auxin effect was further enhanced by adding tryptophan, a precursor of IAA, to the plates and could be mimicked by replacing the Azospirillum cellsby a particular concentration of IAA. Furthermore, particular mutants (rpoN, scrp) and transconjugants (extra copy of ipdC) of A. brasilense were tested in the plate assay. Together, these results confirm the important role of IAA produced by Azospirillum in altering root morphology and illustrate the power of combining genetic tools and bioassays to elucidate the mechanism of a beneficial Azospirillum-plant interaction.
机译:固氮螺菌产生的生长素对观察到的植物生长促进作用很重要。通过使用不同的转基因菌株,在平板试验中评估了巴西柔毛霉生长素在改变根系形态中的生物合成作用。用野生型菌株巴西根霉Sp245和Sp7接种导致根长度强烈减少和根毛形成增加。接种巴西假单胞菌的ipdC突变体后,该效应消失。 ipdC基因编码A. brasilense合成IAA的IPyA途径中的关键酶。另一方面,通过向板中添加IAA的前体色氨酸进一步增强了观察到的植物生长素的作用,并且可以通过用特定浓度的IAA代替偶氮螺旋菌细胞来模仿。此外,在平板测定法中测试了巴西农杆菌的特定突变体(rpoN,scrp)和转导结合体(ipdC的额外拷贝)。总之,这些结果证实了由固氮螺菌产生的IAA在改变根系形态中的重要作用,并说明了将遗传工具和生物测定相结合以阐明有益的固氮菌-植物相互作用的机制的作用。

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