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首页> 外文期刊>Plant and Soil >Root exudation and physiological status of a root-colonizing fluorescent pseudomonad in mycorrhizal and non-mycorrhizal pepper (Capsicum annuum L.).
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Root exudation and physiological status of a root-colonizing fluorescent pseudomonad in mycorrhizal and non-mycorrhizal pepper (Capsicum annuum L.).

机译：菌根和非菌根辣椒（辣椒）中的根定殖荧光假单胞菌的根系分泌物和生理状态。

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Capsicum annuum cv. Yolo Wonder B plants were grown for 27 days in split-root microcosms with one side mycorrhizal with Glomus deserticola (GD) or G. intraradices (GI) while the other side was non-mycorrhizal. Plants with both sides non-mycorrhizalserved as controls. The soil was inoculated with a bioluminescent fluorescent pseudomonad (P. fluorescens 2-79RL). This strain emits light in its exponential growth phase, such that the length of the lag phase prior to bioluminescence can be used to assess the physiological status of the bacterium. Mycorrhizal infection had no significant effect on plant growth. The percentage root length infected was 8% for GD and 34% for GI. After pulse-labelling of the shoots with 14CO2, quartz filter strips were used to collect 14C-labelled root exudates at specific locations on the roots. Compared with the non-mycorrhizal roots, GI decreased 14C-labelled root exudation by 78% at the root tip and by 50% in the older root parts. GD had no effect on 14C-labelled rootexudation. Rhizosphere soil solutions collected with quartz filter strips were analysed for amino acids and organic acids by GC-MS. The overall pattern of the chromatograms of the rhizosphere soil solution was similar in the non-mycorrhizal and the mycorrhizal roots. The number of peaks detected was higher in the non-mycorrhizal roots than in the mycorrhizal roots. Compared with the non-mycorrhizal plants, GI decreased the population density of P. fluorescens 2-79RL on the roots by one order of magnitude, both on the mycorrhizal and the non-mycorrhizal side. GD decreased the population density by one order of magnitude only on the side where the fungus was present. The physiological status of P. fluorescens 2-79RL on the roots, as measured by the length of the lag phase prior to bioluminescence, decreased significantly from day 3 to day 6 and remained at a similar level thereafter. Mycorrhizal infection had little effect on the physiological status. Compared with the non-mycorrhizal plants, GI increased the physiological status of P. fluorescens 2-79RL only during the first 6 days, while GD had no effect at all. It is concluded that mycorrhizal infection may decrease root exudation and alter the composition of the rhizosphere soil solution, therebyreducing the population density of certain bacterial groups in the rhizosphere.
机译：辣椒Yolo Wonder B植物在裂根缩微下生长了27天，一侧菌根与Glomus deserticola（GD）或G. intraradices（GI）菌根，而另一侧则非菌根。两侧均无菌根的植物作为对照。用生物发光的荧光假单胞菌（P.fluorescens 2-79RL）接种土壤。该菌株在其指数生长期发光，因此生物发光之前的滞后阶段的长度可用于评估细菌的生理状态。菌根感染对植物生长没有显着影响。 GD感染根长的百分比为8％，GI感染根长的百分比为34％。用14CO2对芽进行脉冲标记后，使用石英滤纸条在根部的特定位置收集14C标记的根系分泌物。与非菌根相比，GI减少了14C标记的根部分泌物，在根尖减少了78％，在较老的根部减少了50％。 GD对14C标记的根系渗出没有影响。用GC-MS分析用石英滤纸条收集的根际土壤溶液中的氨基酸和有机酸。非菌根和菌根的根际土壤溶液色谱图的总体模式相似。非菌根中检出的峰数高于菌根中的检出峰数。与非菌根植物相比，GI在菌根和非菌根侧均使荧光假单胞菌2-79RL的根系密度降低了一个数量级。 GD仅在存在真菌的一侧将种群密度降低了一个数量级。通过生物发光之前的迟滞期的长度来测量，荧光假单胞菌2-79RL在根上的生理状态从第3天到第6天显着降低并且此后保持在相似水平。菌根感染对生理状态影响很小。与非菌根植物相比，GI仅在头6天内增加了荧光假单胞菌2-79RL的生理状态，而GD则完全没有作用。结论是，菌根感染可减少根系渗出并改变根际土壤溶液的组成，从而降低根际中某些细菌群体的种群密度。

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