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Application of in vitro methods to study carbon uptake and transport by AMfungi

机译：体外方法在AMfungi研究碳吸收和迁移中的应用

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Just as multi-compartmented root chambers have advantages over standard plastic pots for the study of nutrient uptake by arbuscular mycorrhizal [AM] fungi in soil, so the split-plate in vitro system has advantages over the standard dual culture system for the study of the physiology of AM fungi. We used the split-plate culture system of Ri T-DNA transformed Daucus carota L. roots and Glomus intraradices Schenck & Smith, in which only the fungus has access to the distal compartment, to study the ability of germ tubes and extraradical and intraradical hyphae to take up C-13-labeled substrates. Labeled substrates were added to one side of the plate divider and plates were incubated for 8 weeks while the fungus proliferated on the side from which the root was excluded. Tissues then were recovered from the plate and examined via NMR spectroscopy. Results showed that the morphological phases of the fungus differed in their ability to take up these substrates, most notably that intraradical hyphae take up hexose while extraradical hyphae cannot. In addition, NMR studies indicated that intraradical hyphae actively synthesized lipids while extraradical hyphae did not. These data show that eventual axenic culture of AM fungi is more than a matter of finding the proper substrate for growth. Genetic regulation must be overcome to make extraradical hyphae behave like intraradical hyphae in terms of C uptake and metabolism.
机译：就像多室根室在研究土壤中丛枝菌根[AM]真菌对养分的吸收方面优于标准塑料盆一样，离体平板体外系统在研究土壤微生物方面也优于标准双培养系统。 AM真菌的生理学。我们使用了Ri T-DNA转化的胡萝卜（Daucus carota L.）根和Glomus intraradices Schenck＆Smith的分板培养系统，其中只有真菌才能进入远端隔室，以研究胚芽管以及根外和根内菌丝的能力。吸收C-13标记的底物。将标记的底物添加到板分隔器的一侧，并将板孵育8周，同时真菌在排除根的一侧增殖。然后从板中回收组织并通过NMR光谱检查。结果表明，真菌的形态学阶段吸收这些底物的能力不同，最显着的是自由基内的菌丝吸收了己糖，而自由基外的菌丝则不能。此外，NMR研究表明，自由基内的菌丝可以主动合成脂质，而自由基外的菌丝则不能。这些数据表明，最终的AM真菌过氧化物酶培养不仅仅是寻找合适的生长底物。必须克服遗传调控，以使自由基外菌丝在C吸收和代谢方面表现得像自由基内菌丝一样。

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