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首页> 外文期刊>Plant and Soil >MONITORING UPTAKE AND CONTENTS OF MG, CA AND K IN NORWAY SPRUCE AS INFLUENCED BY PH AND AL, USING MICROPROBE ANALYSIS AND STABLE ISOTOPE LABELLING
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MONITORING UPTAKE AND CONTENTS OF MG, CA AND K IN NORWAY SPRUCE AS INFLUENCED BY PH AND AL, USING MICROPROBE ANALYSIS AND STABLE ISOTOPE LABELLING

机译：利用微探针分析和稳定同位素标记监测挪威云杉中镁，钙和钾的摄取和含量

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摘要

In a model system using intact spruce trees (Picea abies [L.] Karst.) we followed the path of magnesium, calcium and potassium during uptake into the root and during long-range transport into the shoot, by multiple stable isotope labelling. The roots of two- and three-year-old spruce trees originating from soil culture were removed from the soil and, in part or in tote, exposed to labelling solutions containing the stable isotopes Mg-25 or Mg-26, K-41 and Ca-42 or Ca-44. Optical-emission-spectroscopy (ICP-OES) of plant fractions and labelling solutions was combined with the quantitative analysis of stable isotope ratios in sections of shock frozen, cryosubstituted material using the laser-microprobe-mass-analyser (LAMMA). This combination allowed us to distinguish, both in bulk samples and on the cellular level between (i) the fraction of elements originally present in the plant before the start of the labelling, (ii) the material taken up from the labelling solution into the plant and (iii) any material released by the plant into the labelling solution.
机译：在使用完整的云杉树木（Picea abies [L.] Karst。）的模型系统中，我们通过多个稳定的同位素标记，在镁吸收，钙吸收和钾吸收，根系吸收以及长距离运输到枝条中的路径。从土壤中取出两岁和三岁的云杉树木的根，将其从土壤中移出，并部分或以手提袋的形式暴露于含有稳定同位素Mg-25或Mg-26，K-41和Ca-42或Ca-44。使用激光微探针质量分析仪（LAMMA），将植物级分和标记溶液的光发射光谱（ICP-OES）与激冷冷冻替代材料的部分中稳定同位素比的定量分析相结合。这种组合使我们能够在大块样品和细胞水平上区分（i）开始标记之前在工厂中最初存在的元素部分，（ii）从标记溶液吸收到工厂中的物质（iii）植物释放到标签溶液中的任何材料。

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