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首页> 外文期刊>Plant and Soil >Soil microbial community responses to altered lignin biosynthesis in Populus tremuloides vary among three distinct soils.
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Soil microbial community responses to altered lignin biosynthesis in Populus tremuloides vary among three distinct soils.

机译：在三种不同的土壤中，土壤微生物群落对木质杨生物合成改变的响应有所不同。

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摘要

The development and use of transgenic plants has steadily increased, but there are still little data about the responses of soil microorganisms to these genetic modifications. We utilized a greenhouse trial approach to evaluate the effects of altered stem lignin in trembling aspen (Populus tremuloides) on soil microbial communities in three soils which differed in their chemical and physical properties; they included a sandy loam (CO-Colorado), a silt loam (KS-Kansas), and a clay loam (TX-Texas). Three transgenic aspen lines were developed from a natural clone common to the Great Lakes region of North America. The concentrations of stem lignin concentrations were reduced by 35% (Line 23), 40% (Line 141) and 50% (Line 72). Line 72 and Line 141 also had a 40 and 20% increase in syringyl-type stem lignin, respectively. Indirectly, these modifications resulted in increased (5-13%) and decreased (-5 to -57%) levels of root production across the lines and soil types. Responses of the soil microbial communities were investigated using: phospholipid fatty acids (PLFA), neutral lipid fatty acids (NLFA), and (3) extracellular enzyme assays. PLFA analyses indicated that there were large differences in microbial community composition between the three soils. Similarly, there were large differences in total NLFA between soils, with the KS soils having the highest amount and CO the lowest. Enzyme activities did not differ between soils, except for cellubiohydrolase, which was highest in CO soil. Across all three soils, responses to the four genetic lines were not consistent. Interactions between soil type and genetic line make it difficult to assess the potential ecological impacts of transgenic aspen on soil microbial communities and their associated functions. Given these interactions, field trials with transgenic aspen should encompass the wide range of soils targeted for commercial planting in order to determine their effect(s) on the resident soil microbial community.
机译：转基因植物的开发和使用稳步增长，但是关于土壤微生物对这些基因修饰的反应的数据仍然很少。我们利用温室试验方法评估了颤抖的白杨（木杨）中改变的木质素对三种化学和物理性质不同的土壤微生物群落的影响。其中包括沙质壤土（科罗拉多州科罗拉多州），粉质壤土（堪萨斯州堪萨斯州）和黏土壤土（德克萨斯州德克萨斯州）。从北美大湖地区常见的天然克隆中开发了三种转基因白杨系。茎木质素浓度降低了35％（第23行），40％（第141行）和50％（第72行）。品系72和品系141的丁香基型茎木质素也分别增加了40％和20％。间接地，这些修饰导致各系和土壤类型的根系生产水平增加（5-13％）和减少（-5至-57％）。使用以下方法研究了土壤微生物群落的响应：磷脂脂肪酸（PLFA），中性脂质脂肪酸（NLFA）和（3）细胞外酶法。 PLFA分析表明，三种土壤之间的微生物群落组成存在很大差异。同样，土壤之间的总NLFA也有很大差异，KS土壤含量最高，而CO含量最低。除纤维二糖水解酶外，土壤中的酶活性没有差异，后者在CO土壤中最高。在所有三种土壤中，对四种遗传系的反应均不一致。土壤类型和遗传系之间的相互作用使得很难评估转基因白杨对土壤微生物群落及其相关功能的潜在生态影响。鉴于这些相互作用，转基因白杨的田间试验应涵盖针对商业化种植的多种土壤，以确定它们对居民土壤微生物群落的影响。

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