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Gliadins polymorphism and cluster analyses of syrian grown durum wheat

机译：叙利亚硬质小麦的麦醇溶蛋白多态性和聚类分析

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A total of 187 Triticum durum (Desf.) genotypes were studied. These included 102 mutants, 15 local genotypes, 22 lines from ACSAD and 48 lines from ICARDA. Polyacrylamide gel electrophoresis under acidic conditions of pH 3.1 (A-PAGE) was used to separate gliadins groups of storage proteins for the identification and the classification of the genotypes under study. Results showed that the region of omega-gliadins had a wider range for the number of bands than all other regions of gliadins (alpha-, beta-, and gamma-gliadins). Cluster analyses using the Unweighted Pair Group Mean Average (UPGMA) method put the genotypes of all groups in trees on the basis of the gliadin bands distribution. Three categories were obtained: 1st - complete correspondenceof the pedigrees and the trees, reflecting the importance of the gliadins as a decisive factor for the genotype position in the cluster; 2nd - the presence of genotypes with similar banding patterns but were unrelated in their pedigrees; 3rd - the genotypes originate from the same cross but are unrelated in the tree. It was concluded that tree clustering based on gliadin electrophoregrams may be used as an additional tool in revealing genetical relations among genotypes. However, one should keep in mindthat several factors may influence the resulting tree. These include heterogeneity, incorrect band designation and uncertain or false pedigrees.
机译：总共研究了187个硬粒小麦（Desf。）基因型。这些包括102个突变体，15个局部基因型，来自ACSAD的22个系和来自ICARDA的48个系。在酸性条件下（pH 3.1）进行聚丙烯酰胺凝胶电泳（A-PAGE）以分离贮藏蛋白的麦醇溶蛋白基团，以鉴定和分类所研究的基因型。结果显示，与所有其他麦醇溶蛋白区域（α-，β-和γ-麦醇溶蛋白）相比，欧米茄-麦醇溶蛋白区域的谱带数目范围更广。使用非加权对平均组（UPGMA）方法进行的聚类分析基于麦醇溶蛋白带的分布，将树木中所有组的基因型置于基因组中。获得了三类：第一-谱系和树木的完全对应，反映了麦醇溶蛋白是决定簇中基因型位置的决定性因素的重要性; 2-存在具有相似带型的基因型，但在血统谱系上不相关;第三-基因型来自同一杂交种，但在树中无关。结论是，基于麦醇溶蛋白电泳图的树簇可以用作揭示基因型之间遗传关系的附加工具。但是，请记住，有几个因素可能会影响生成的树。这些包括异质性，不正确的频段指定以及不确定或错误的血统书。

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