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首页> 外文期刊>Plant Breeding and Seed Science >USE OF AFLP MOLECULAR MARKERS FOR ESTIMATING GENETIC SIMILARITY OF ALFALFA (MEDICAGO SATIVA L. SL.)
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USE OF AFLP MOLECULAR MARKERS FOR ESTIMATING GENETIC SIMILARITY OF ALFALFA (MEDICAGO SATIVA L. SL.)

机译：使用AFLP分子标记估算苜蓿的遗传相似性（MEDICAGO SATIVA L. SL。）

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摘要

The aim of study was to determine genetic similarity among sixteen alfalfa populations using the AFLP technique. Plant material was selected considering the high genotypic variability. It included populations of different origin: native forms of alfalfa (secondary ecotypes), inbred lines, single hybrids, synthetic populations, varieties and mutants. Analysis was carried out using 10 combinations of primers. It was noticed, that in case of the studied alfalfa populations, the highest polymorphism wasobtained using primers with three selective nucleotides. The genetic similarity coefficients were calculated by the method of Nei and Li. Those coefficients were used for the hierarchical grouping of objects by the unweighted pair group method of arithmetic means. Results of the grouping were presented in the form of dendrogram. Values of coefficients ranged from 0.3311 (between the inbred F line and the Ulstar variety) to 0.8076 (between ecotype 27 and the synthetic population Syn 7-3). Dendrogram of genetic similarity obtained in this way did not fully correspond with the known data about the studied populations' origin. Considering the genetic similarity, inbreed line F was the most outlying from the studied populations of alfalfa.
机译：研究的目的是使用AFLP技术确定16个苜蓿种群之间的遗传相似性。考虑到高基因型变异性，选择植物材料。它包括不同来源的种群：苜蓿的天然形式（次生生态型），近交系，单一杂种，合成种群，品种和突变体。使用10种引物组合进行分析。注意到，在所研究的苜蓿种群中，使用具有三个选择性核苷酸的引物获得了最高的多态性。用Nei和Li的方法计算遗传相似性系数。这些系数通过算术方法的非加权对组方法用于对象的分层分组。分组结果以树状图的形式显示。系数的值介于0.3311（近交F系和Ulstar品种之间）至0.8076（介于生态型27和合成种群Syn 7-3之间）之间。以这种方式获得的遗传相似性的树状图与所研究人群的起源的已知数据并不完全一致。考虑到遗传相似性，近交系F是苜蓿研究种群中最不远的一个。

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