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首页> 外文期刊>Plant biology >Molecular cloning and expression of a cDNA encoding a hybrid histidine kinase receptor in tropical periwinkle Catharanthus roseus
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Molecular cloning and expression of a cDNA encoding a hybrid histidine kinase receptor in tropical periwinkle Catharanthus roseus

机译：热带长春花长春花的分子克隆及编码杂合组氨酸激酶受体的cDNA的表达

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摘要

Signalling pathways involving histidine kinase receptors (HKRs) are widely used by prokaryotes and fungi to regulate a large palette of biological processes. in plants, HKRs are known to be implicated in cytokinin, ethylene, and osmosensing transduction pathways. In this work, a full length cDNA named CrClK was isolated from the tropical species Catharanthus roseus (L.) G. Don. It encodes a 1205 amino acid protein that belongs to the hybrid HKR family. The deduced amino acid sequence shows the highest homology with AtHK1, an osmosensing HKR in Arabidopsis thaliana. In return, CrClK protein shares very low identity with the other 10 Arabidopsis HKRs. Southern blot analysis indicates that the CrClK corresponding gene is either present in multiple copies or has very close homologues in the genome of the tropical periwinkle. The gene is widely expressed in the plant. In C. roseus C20D cell suspension, it is slightly induced after exposure to low temperature, pointing to a putative role in cold-shock signal transduction.
机译：涉及组氨酸激酶受体（HKR）的信号传导途径被原核生物和真菌广泛用于调节大量的生物过程。在植物中，HKR已知与细胞分裂素，乙烯和渗透压传导通路有关。在这项工作中，从热带物种Catharanthus roseus（L.）G. Don。分离出了一个名为CrClK的全长cDNA。它编码属于杂交HKR家族的1205个氨基酸蛋白。推导的氨基酸序列与拟南芥中的渗透性HKR AtHK1具有最高的同源性。作为回报，CrClK蛋白与其他10个拟南芥HKR具有极低的同一性。 Southern印迹分析表明，在热带长春花的基因组中，CrClK相应的基因以多拷贝存在或具有非常接近的同源物。该基因在植物中广泛表达。在玫瑰色念珠菌C20D细胞悬浮液中，暴露于低温后会被轻微诱导，这表明在冷休克信号转导中可能发挥了作用。

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