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首页> 外文期刊>Plant Cell Reports >Genetic transformation of cowpea (Vigna unguiculata L.) and stable transmission of the transgenes to progeny
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Genetic transformation of cowpea (Vigna unguiculata L.) and stable transmission of the transgenes to progeny

机译：of豆（Vigna unguiculata L.）的遗传转化和转基因稳定地传给子代

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Cowpeas are nutritious grains that provide the main source of protein, highly digestible energy and vitamins to some of the world's poorest people. The demand for cowpeas is high but yields remain critically low, largely because of insect pests. Cowpea germplasm contains little or no resistance to major insect pests and a gene technology approach to adding insect protection traits is now a high priority. We have adapted features of several legume and other transformation systems and reproducibly obtained transgenic cowpeas that obey Mendelian rules in transmitting the transgene to their progeny. Critical parameters in this transformation system include the choice of cotyledonary nodes from developing or mature seeds as explants and a tissue culture medium devoid of auxins in the early stages, but including the cytokinin BAP at low levels during shoot initiation and elongation. Addition of thiol-compounds during infection and co-culture with Agrobacterium and the choice of the bar gene for selection with phosphinothricin were also important. Transgenic cowpeas that transmit the transgenes to their progeny can be recovered at a rate of one fertile plant per thousand explants. These results pave the way for the introduction of new traits into cowpea and the first genes to be trialled will include those with potential to protect against insect pests.
机译：豆是一种营养谷物，可为世界上一些最贫穷的人们提供蛋白质，高消化率的能量和维生素的主要来源。对cow豆的需求很高，但单产仍然非常低，主要原因是害虫。豆种质对主要害虫几乎没有抗药性，几乎没有抗药性，现在，增加害虫保护性状的基因技术方法已成为当务之急。我们适应了几种豆科植物和其他转化系统的特征，并以可再生的方式获得了转基因cow豆，这些转基因cow豆在将转基因传递给后代时遵循孟德尔规则。该转化系统中的关键参数包括从发育中的种子或成熟种子中选择子叶节作为外植体，以及在早期没有生长素的组织培养基，但在芽生和伸长过程中细胞分裂素BAP含量较低。在与土壤杆菌的感染和共培养过程中添加硫醇化合物以及选择与膦丝菌素一起选择的bar基因也很重要。可以将转基因传递给后代的转基因can豆的回收率是每千个外植体中有一种可育植物。这些结果为将新性状引入cow豆铺平了道路，将要试验的第一个基因将包括具有潜在的防虫能力的基因。

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