...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >High-frequency generation and characterization of intergeneric hybrids and haploids from new wheat-barley crosses
【24h】

High-frequency generation and characterization of intergeneric hybrids and haploids from new wheat-barley crosses

机译：小麦-大麦新杂交种的杂种和单倍体的高频产生和鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hybrid plants and a high frequency of maternal haploids were obtained using an efficient wheat-barley hybridization system (with new genotype combinations) and confirmed by several cytological and molecular tools. An efficient hybridization system between wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) is presented on the basis of three new genotype combinations. A particularly high, 14 % frequency of plant regeneration per florets was achieved in the wheat-barley genotype combination of 'Sichuan' x 'Morex'. The genome composition in 42 of the 95 plants regenerated by embryo rescue was determined using ploidy analysis, genomic in situ hybridization and the application of chromosome arm-specific molecular markers (SSR and STS). A high overall frequency (76 %) of maternal (wheat) haploids was observed in all the tests for all three cross combinations. A major implication of this observation is that this new hybridization system represents a useful tool to study the mechanism of uniparental chromosome elimination in cereals
机译：使用高效的小麦-大麦杂交系统（具有新的基因型组合）获得了杂种植物和高频率的母本单倍体，并通过多种细胞学和分子工具进行了证实。基于三种新的基因型组合，提出了小麦（Triticum aestivum L.）和大麦（Hordeum vulgare L.）之间的高效杂交系统。在“四川”ד Morex”的小麦-大麦基因型组合中，每小花的植株再生频率特别高，达到14％。通过倍性分析，基因组原位杂交以及染色体臂特异性分子标记（SSR和STS）的应用，确定了通过胚胎拯救再生的95种植物中42种的基因组组成。在所有三个交叉组合的所有测试中，母体（小麦）单倍体的总频率很高（76％）。该观察结果的主要含义是，这种新的杂交系统代表了研究谷物单亲染色体消除机制的有用工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号