Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.

Wang Y; Qiu L; Dai C; Wang J; Luo J; Zhang F; Ma J

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Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.

机译：昆虫（Microdera puntipennis dzungarica）抗冻蛋白MpAFP149的表达赋予转基因烟草耐寒性。

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To elucidate the function of antifreeze protein from Microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of MpAFP149 gene with the signal peptide sequence responsible for secreting the native MpAFP149 into the apoplast space under control of a cauliflower mosaic virus 35S promoter was introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. The observation of immunogold localization by TEM (transmission electron microscope) showed that the heterologous MpAFP149 protein was mainly distributed on the cell wall in apoplast of the transgenic tobacco plant. T1 generation transgenic tobacco plants displayed a more frost resistant phenotype and kept the lower ion leakage ratio and MDA (malondialdehyde) content in the leaves compared with wild-type ones at -1 degrees C for 3 days. The results showed that MpAFP149 provided protection and conferred cold tolerance to transgenic tobacco plant during freezing stress.

机译：为了阐明Microdera puntipennis dzhungarica的抗冻蛋白在植物中耐低温胁迫的功能，将具有信号肽序列的MpAFP149基因构建体引入了在花椰菜花叶病毒35S启动子的控制下将天然MpAFP149分泌到质外体空间中的功能。根癌农杆菌介导转化烟草。通过透射电子显微镜（TEM）观察免疫金的定位，发现异源MpAFP149蛋白主要分布在转基因烟草植物质外体的细胞壁上。与野生型烟草相比，T1代转基因烟草植物在-1摄氏度的环境中表现出更高的抗冻表型，并在-1摄氏度下保持了较低的离子泄漏率和MDA（丙二醛）含量。结果表明，MpAFP149在冰冻胁迫下为转基因烟草植物提供了保护并赋予了其耐寒性。

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《Plant Cell Reports》 |2008年第8期|共10页
作者
Wang Y; Qiu L; Dai C; Wang J; Luo J; Zhang F; Ma J;
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中图分类植物细胞学;
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1. Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco. [J] . Wang Y, Qiu L, Dai C, Plant Cell Reports . 2008,第8期

机译：昆虫（Microdera puntipennis dzungarica）抗冻蛋白MpAFP149的表达赋予转基因烟草耐寒性。
2. Hyperactive spruce budworm antifreeze protein expression in transgenic Drosophila does not confer cold shock tolerance. [J] . Tyshenko MG, Walker VK Cryobiology: International Journal of Low Temperature Biology and Medicine . 2004,第1期

机译：高活性云杉芽虫抗冻蛋白在转基因果蝇中的表达不赋予抗寒性。
3. ANTIFREEZE PROTEIN DOES NOT CONFER COLD TOLERANCE TO TRANSGENIC DROSOPHILA MELANOGASTER [J] . Duncker BP, Chen CP, Davies PL, Cryobiology: International Journal of Low Temperature Biology and Medicine . 1995,第6期

机译：抗冻蛋白不能赋予转基因果蝇黑变种耐寒性
4. Expression of both SOD and APX genes confers multiple abiotic stress tolerance to transgenic tall fescue plants [C] . LEE Sang-Hoon, WON Sung-Hye, KWON Suk-Yoon, Acta Prataculturae Sinica vol.15 Suppl. July 2006: Grassland Agriculture: Balancing Production and Environmental Protection . 2006

机译：SOD和APX基因的表达赋予转基因高羊茅植物多种非生物胁迫耐受性
5. Insect antifreeze proteins and the expression and characterization of spruce budworm isoforms in transgenic systems. [D] . Tyshenko, Michael G. 2004

机译：昆虫抗冻蛋白以及云杉芽虫同工型在转基因系统中的表达和特征。
6. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold dehydration and salt stress in transgenic tobacco [O] . Arnab Mukhopadhyay, Shubha Vij, Akhilesh K. Tyagi 2004

机译：水稻中锌指蛋白基因的过量表达赋予转基因烟草耐寒脱水和盐分胁迫的能力
7. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco [O] . Mukhopadhyay, Arnab, Vij, Shubha, Tyagi, Akhilesh K. 2004

机译：水稻中锌指蛋白基因的过量表达赋予转基因烟草耐寒，脱水和盐分胁迫的能力

Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.

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