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首页> 外文期刊>Plant Cell Reports >Constitutive expression of a meiotic recombination protein gene homolog, OsTOP6A1, from rice confers abiotic stress tolerance in transgenic Arabidopsis plants
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Constitutive expression of a meiotic recombination protein gene homolog, OsTOP6A1, from rice confers abiotic stress tolerance in transgenic Arabidopsis plants

机译：水稻减数分裂重组蛋白基因同源物OsTOP6A1的组成型表达赋予转基因拟南芥植物非生物胁迫耐受性

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摘要

Plant productivity is greatly influenced by various environmental stresses, such as high salinity and drought. Earlier, we reported the isolation of topoisomerase 6 homologs from rice and showed that over expression of OsTOP6A3 and OsTOP6B confers abiotic stress tolerance in transgenic Arabidopsis plants. In this study, we have assessed the function of nuclear-localized topoisomerase 6 subunit A homolog, OsTOP6A1, in transgenic Arabidopsis plants. The over expression of OsTOP6A1 in transgenic Arabidopsis plants driven by cauliflower mosaic virus-35S promoter resulted in pleiotropic effects on plant growth and development. The transgenic Arabidopsis plants showed reduced sensitivity to stress hormone, abscisic acid (ABA), and tolerance to high salinity and dehydration at the seed germination; seedling and adult stages as reflected by the percentage of germination, fresh weight of seedlings and leaf senescence assay, respectively. Concomitantly, the expression of many stress-responsive genes was enhanced under various stress conditions in transgenic Arabidopsis plants. Moreover, microarray analysis revealed that the expression of a large number of genes involved in various processes of plant growth and development and stress responses was altered in transgenic plants. Although AtSPO11-1, the homolog of OsTOP6A1 in Arabidopsis, has been implicated in meiotic recombination; the present study demonstrates possible additional role of OsTOP6A1 and provides an effective tool for engineering crop plants for tolerance to different environmental stresses.
机译：植物生产力在很大程度上受到各种环境压力的影响，例如高盐度和干旱。早些时候，我们报道了从水稻中分离拓扑异构酶6的同源物，并表明OsTOP6A3和OsTOP6B的过度表达赋予转基因拟南芥植物以非生物胁迫耐受性。在这项研究中，我们评估了转基因拟南芥植物中核定位的拓扑异构酶6亚基A同源物OsTOP6A1的功能。花椰菜花叶病毒35S启动子驱动的转基因拟南芥植物中OsTOP6A1的过表达导致多效性对植物生长发育的影响。转基因拟南芥植株对胁迫激素，脱落酸（ABA）的敏感性降低，并且对种子萌发时高盐度和脱水的耐受性增强。分别通过发芽率，幼苗鲜重和叶片衰老试验反映出苗期和成年期。相应地，在各种胁迫条件下，转基因拟南芥植物中许多胁迫响应基因的表达均得到增强。此外，微阵列分析显示，在转基因植物中，参与植物生长发育和胁迫反应的大量基因的表达发生了变化。尽管AtSPO11-1是拟南芥中OsTOP6A1的同源物，但它参与了减数分裂重组。本研究证明了OsTOP6A1可能还有其他作用，并为工程作物植物耐受不同环境胁迫提供了有效的工具。

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