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首页> 外文期刊>Plant Cell Reports >Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).
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Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).

机译：从毛竹（ Dendrocalamus latiflorus Munro）幼叶的标准化cDNA文库中生成和分析表达的序列标签。

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摘要

Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A normalized cDNA library was constructed from young leaves of Ma bamboo and 9,574 high-quality ESTs were generated, from which 5,317 unigenes including 1,502 contigs and 3,815 singletons were assembled. The unigenes were assigned into different gene ontology (GO) categories and summarized into 13 broad biologically functional groups according to similar functional characteristics or cellular roles by BLAST search against public databases. Eight hundred and ninety-one unigenes were assigned by KO identifiers and mapped to six KEGG biochemical pathways. The transcripts involved in biosynthesis of secondary metabolites such as cytochrome 450, flavonol synthase/flavanone 3-hydroxylase, and dihydroflavonol-4-reductase were well represented by 14 unigenes in the unigene set. The candidate genes involved in phytohormone metabolism, signal transduction and encoding cell wall-associated receptor kinases were also identified. Sixty-seven unigenes related to plant resistance (R) genes, including RPP genes, RGAs and RDL/RF genes, were discovered. These results will provide genome-wide knowledge about the molecular physiology of Ma bamboo young leaves and tools for advanced studies of molecular mechanism underlying leaf growth and development.Digital Object Identifier http://dx.doi.org/10.1007/s00299-011-1112-0
机译：麻竹（ Dendrocalamus latiflorus Munro）属于 Dendrocalamus 属， Bambusease 部落， Bambusoideae 子科，禾本科家庭。它是成簇的竹的代表种，并且是使用成熟的茎秆用于各种建筑目的和使用幼芽供人类食用的主要商业品种。用毛竹的幼叶构建了一个标准化的cDNA文库，并产生了9,574个高质量的EST，从中组装了5,317个单基因，包括1,502个重叠群和3,815个单子。通过对公共数据库进行BLAST搜索，根据相似的功能特征或细胞作用，将单基因分为不同的基因本体（GO）类别，并归纳为13个广泛的生物学功能组。 KO标识符分配了819个单基因，并映射到6条KEGG生化途径。在单基因组中有14个单基因很好地代表了次级代谢产物如细胞色素450，黄酮醇合酶/黄酮3-羟化酶和二氢黄酮醇4-还原酶的生物合成中的转录本。还鉴定了参与植物激素代谢，信号转导和编码细胞壁相关受体激酶的候选基因。发现了与植物抗性（R）基因相关的67个单基因，包括RPP基因，RGA和RDL / RF基因。这些结果将为全基因组知识提供有关毛竹幼叶的分子生理学知识以及用于深入研究叶生长和发育的分子机制的工具。数字对象标识符http://dx.doi.org/10.1007/s00299-011- 1112-0

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