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首页> 外文期刊>Plant Cell Reports >Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.)
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Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.)

机译：水稻中雌激素诱导的GFP表达模式

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摘要

We investigated estrogen-inducible green fluorescent protein (GFP) expression patterns using an estrogen receptor fused chimeric transcription activator, XVE, in the monocotyledonous model plant rice (Oryza sativa L.). This system has been shown to be an effective chemical-inducible gene expression system in Arabidopsis and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, pLex:GFP, and investigated dose-response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-o-estradiol treatment at >5 oM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 oM. In three-leaf-stage seedlings, GFP signals in the leaves of pLex:GFP-integrated transgenic lines were weaker than those in the leaves of p35S:GFP-integrated transgenic lines. However, GFP signals in the roots of pLex:GFP- and p35S:GFP-integrated transgenic lines were similar with estradiol treatment at >10 oM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.
机译：我们在单子叶植物模型水稻（Oryza sativa L.）中研究了使用雌激素受体融合的嵌合转录激活剂XVE研究雌激素诱导的绿色荧光蛋白（GFP）的表达模式。该系统已被证明是拟南芥中一种有效的化学诱导基因表达系统，并已应用于其他植物以研究基因功能或生产无标记转基因植物。但是，关于诱导子浓度与单子叶植物中诱导的基因表达水平之间的相关性的信息有限。在这里，我们生产了转基因水稻整合的雌激素诱导型GFP表达载体pLex：GFP，并首次研究了水稻愈伤组织和幼苗中GFP诱导的剂量反应和时程模式。用> 5 oM的17-o-雌二醇处理，在培养2天之内在整个愈伤组织表面检测到GFP信号。最高的GFP信号在25 oM的雌二醇处理下延长了8天。在三叶期幼苗中，pLex：GFP整合的转基因系叶片中的GFP信号弱于p35S：GFP整合的转基因系叶片中的GFP信号。然而，pLex：GFP和p35S：GFP整合的转基因品系的根中的GFP信号与> 10 oM的雌二醇处理相似。关于控制适当的基因表达，这些结果可能为水稻和其他单子叶植物的XVE系统使用的雌二醇处理条件提供有用的指示。

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