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首页> 外文期刊>Plant Cell Reports >Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation
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Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation

机译：氯化钾和稀土元素改善植物生长并增加根癌农杆菌介导的植物转化的频率

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摘要

Plant transformation efficiency depends on the ability of the transgene to successfully interact with plant host factors. Our previous work and the work of others showed that manipulation of the activity of host factors allows for increased frequency of transformation. Recently we reported that exposure of tobacco plants to increased concentrations of ammonium nitrate increases the frequency of both homologous recombination and plant transgenesis. Here we tested the influence of KCl and salts of rare earth elements, Ce and La on the efficiency of Agrobacterium-mediated plant transformation. We found that exposure to KCl, CeCl and LaCl leads to an increase in recombination frequency in Arabidopsis and tobacco. Plants grown in the presence of CeCl and LaCl had higher biomass, longer roots and greater root number. Analysis of transformation efficiency showed that exposure of tobacco plants to 50 mM KCl resulted in ~6.0-fold increase in the number of regenerated calli and transgenic plants as compared to control plants. Exposure to various concentrations of CeCl showed a maximum increase of ~3.0-fold in both the number of calli and transgenic plants. Segregation analysis showed that exposure to KCl and cerium (III) chloride leads to more frequent integrations of the transgene at a single locus. Analysis of transgene intactness showed better preservation of right T-DNA border during transgene integration. Our data suggest that KCl and CeCl can be effectively used to improve quantity and quality of transgene integrations.
机译：植物转化效率取决于转基因与植物宿主因子成功相互作用的能力。我们之前的工作和其他人的工作表明，操纵宿主因子的活动可以增加转化的频率。最近，我们报道了烟草植物暴露于浓度更高的硝酸铵中会增加同源重组和植物转基因的频率。在这里，我们测试了氯化钾和稀土元素，铈和镧的盐对农杆菌介导的植物转化效率的影响。我们发现暴露于KCl，CeCl和LaCl会导致拟南芥和烟草中重组频率增加。在存在CeCl和LaCl的条件下生长的植物具有更高的生物量，更长的根和更大的根数。转化效率的分析表明，与对照植物相比，将烟草植物暴露于50 mM KCl可使再生愈伤组织和转基因植物的数量增加约6.0倍。暴露于各种浓度的CeCl中，愈伤组织和转基因植物的数量最多增加约3.0倍。分离分析表明，暴露于KCl和氯化铈（III）会导致转基因在单个位点更频繁地整合。对转基因完整性的分析表明，在转基因整合过程中，更好地保留了正确的T-DNA边界。我们的数据表明，KCl和CeCl可以有效地用于提高转基因整合的数量和质量。

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