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首页> 外文期刊>Plant Cell Reports >Expression of the major mugwort pollen allergen Art v 1 in tobacco plants and cell cultures: problems and perspectives for allergen production in plants. (Special Issue: Plant molecular pharming 2012 and beyond.)
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Expression of the major mugwort pollen allergen Art v 1 in tobacco plants and cell cultures: problems and perspectives for allergen production in plants. (Special Issue: Plant molecular pharming 2012 and beyond.)

机译：主要艾蒿花粉过敏原Art v 1在烟草植物和细胞培养物中的表达：植物过敏原产生的问题和前景。 （特刊：2012年及以后的植物分子药物开发。）

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摘要

An economic and cheap production of large amounts of recombinant allergenic proteins might become a prerequisite for the common use of microarray-based diagnostic allergy assays which allow a component-specific diagnosis. A molecular pharming strategy was applied to express the major allergen of Artemisia vulgaris pollen, Art v 1, in tobacco plants and tobacco cell cultures. The original Art v 1 with its endogenous signal peptide which directs Art v 1 to the secretory pathway, was expressed in transiently transformed tobacco leaves but was lost in stable transformed tobacco plants during the alternation of generations. Using a light-regulated promoter and "hiding" the recombinant Art v 1 in the ER succeeded in expression of Art v 1 over three generations of tobacco plants and in cell cultures generated from stable transformed plants. However, the amounts of the recombinant allergen were sufficient for analysis but not high enough to allow an economic production. Although molecular pharming has been shown to work well for the production of non-plant therapeutic proteins, it might be less efficient for closely related plant proteins.
机译：经济和廉价地生产大量的重组变应原蛋白可能成为普遍使用基于微阵列的诊断变态反应检测的先决条件，这种方法可进行特定组分的诊断。应用分子药物化策略在烟草植物和烟草细胞培养物中表达寻常蒿花粉Art v 1的主要变应原。最初的Art v 1及其内源信号肽将Art v 1引导至分泌途径，在瞬时转化的烟叶中表达，但在世代交替中在稳定的转化烟草植物中丢失。使用光调节的启动子并“隐藏”在ER中的重组Art v 1成功地在三代烟草植物中表达了Art v 1，并在由稳定的转化植物产生的细胞培养物中成功表达了Art v 1。但是，重组变应原的量足以进行分析，但不足以进行经济生产。尽管已经证明分子修饰对于生产非植物性治疗性蛋白质非常有效，但对于紧密相关的植物性蛋白质可能效率较低。

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