• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >Microarray analysis of differentially expressed genes between Brassica napus strains with high- and low-oleic acid contents.
【24h】

Microarray analysis of differentially expressed genes between Brassica napus strains with high- and low-oleic acid contents.

机译：甘蓝型油菜高油酸含量和低油酸含量之间差异表达基因的微阵列分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An increase in oleic acid (C18:1) content is a desirable trait. Despite the critical roles of the two desaturases, FAD2 and FAD3, in the control of fatty acid desaturation, a dispute remains over whether inactivation of their genes alone is sufficient enough to generate the high-oleic trait. To address this question, we employed microarray technology to investigate the difference in gene expression profile between two different Brassica napus strains with high-C18:1 (71.71%) and low-C18:1 (55.6%) contents, respectively. Our study revealed 562 differentially expressed genes, of which 194 genes were up-regulated and 368 down-regulated. Based on the Gene Ontology classification, these genes were classified into 23 functional categories. Three of the up-regulated genes represent B. napus homologs of Arabidopsis genes encoding a cytosolic isoform of pyruvate kinase (AT3G55810), Delta9 acyl-lipid desaturase (AT1G06080, ADS1) and fatty acyl-ACP thioesterase B (AT1G08510), respectively. Conversely, the homologs of two Arabidopsis sequences encoding Delta9 acyl-lipid desaturase (AT2G31360, ADS2) and FAD3 desaturase (AT2G29980) were down-regulated in the high-oleic acid strain. Furthermore, 60 differentially expressed genes were classified as associated with relevant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Collectively, our results suggest that expressing the high-oleic acid trait may require a coordinated regulation of diverse regulatory and metabolic gene networks in addition to inactivation of the FAD2 and FAD3 genes in the oilseed. A set of the differentially expressed genes identified in this study will facilitate our efforts to tap the germplasms with the potential to express the high-oleic acid trait
机译：油酸（C18：1）含量的增加是理想的特性。尽管两种去饱和酶FAD2和FAD3在控制脂肪酸去饱和中起着关键作用，但仅它们的基因失活是否足以产生高油酸性状仍存在争议。为了解决这个问题，我们采用微阵列技术研究了高C18：1（71.71％）和低C18：1（55.6％）的两个不同甘蓝型油菜菌株之间基因表达谱的差异。内容。我们的研究揭示了562个差异表达基因，其中194个基因被上调而368个基因被下调。根据基因本体论分类，这些基因被分为23个功能类别。上调的基因中的三个代表B。拟南芥基因的napus同源，分别编码丙酮酸激酶（AT3G55810），Delta9酰基脂质去饱和酶（AT1G06080，ADS1）和脂肪酰基-ACP硫酯酶B（AT1G08510）的胞质亚型。相反，在高油酸菌株中，两个编码Delta9酰基脂质去饱和酶（AT2G31360，ADS2）和FAD3去饱和酶（AT2G29980）的拟南芥序列的同源物被下调。此外，将60个差异表达的基因归类为与《京都议定书》相关的基因与基因组百科全书（KEGG）途径。总体而言，我们的研究结果表明，表达高油酸性状可能需要除使 FAD2 和 FAD3 基因失活外，还需要对各种调控和代谢基因网络进行协调调控。油籽。本研究中鉴定的一组差异表达基因将有助于我们努力开发具有表达高油酸性状潜力的种质

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号