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首页> 外文期刊>Plant Cell Reports >DFL, a FLORICAULA/LEAFY homologue gene from Dendranthema lavandulifolium is expressed both in the vegetative and reproductive tissues
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DFL, a FLORICAULA/LEAFY homologue gene from Dendranthema lavandulifolium is expressed both in the vegetative and reproductive tissues

机译：DFL是一种来自淡紫色石end的FLORICAULA / LEAFY同源基因，在营养和生殖组织中均表达

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摘要

FLO/LFY homologue genes were initially characterized as floral meristem identity genes and play a key role in flower development among diverse species. The inflorescence organization of chrysanthemum differs from typical dicotyledons such as Arabidopsis and Antirrhinum as clear sepals are absent, and instead, a pappus, a rudimentary sepal, is formed. To understand the mechanism of reproduction of chrysanthemum at the molecular level, DFL, a FLORICAULA/LEAFY homologous gene, was cloned from Dendranthema lavandulifolium, which is one of the original species of chrysanthemum. The DFL gene consists of a 1,236-bp open reading frame and encodes a putative protein of 412 amino acids, which is 63% identical to LFY and 70% to FLO. The expression patterns of DFL during the flower development were analyzed, and RT-PCR results showed that DFL was strongly expressed in the flower bud. In situ hybridization experiments showed that it is strongly expressed in the inflorescence bract, petal and stamen primordial tissues throughout the inflorescence development. Its expression signals were also detected in stems, leaf primordial tissues and developing inflorescence bracts.
机译：FLO / LFY同源基因最初被表征为花分生组织同一性基因，并且在不同物种之间的花发育中起关键作用。菊花的花序组织不同于典型的双子叶植物，如拟南芥和Antirrhinum，因为缺少透明的萼片，相反，形成了一个乳头，即原始的萼片。为了了解菊花在分子水平上的繁殖机理，从菊花的原始种之一薰衣草（Dendranthema lavandulifolium）中克隆了一种FLORICAULA / LEAFY同源基因DFL。 DFL基因包含一个1,236 bp的开放阅读框，编码412个氨基酸的推定蛋白质，与LFY同源性为63％，与FLO同源性为70％。分析了花发育过程中DFL的表达模式，RT-PCR结果表明DFL在花芽中强烈表达。原位杂交实验表明，它在整个花序发育过程中在is片，花瓣和雄蕊原始组织中强烈表达。在茎，叶原始组织和发育中的花序片中也检测到其表达信号。

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