• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >The constitutive expression of Chrysanthemum dichrum ICE1 in Chrysanthemum grandiflorum improves the level of low temperature, salinity and drought tolerance.
【24h】

The constitutive expression of Chrysanthemum dichrum ICE1 in Chrysanthemum grandiflorum improves the level of low temperature, salinity and drought tolerance.

机译：菊花ICE1在大花菊花中的组成型表达提高了低温，盐分和耐旱性水平。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved bHLH domain, a nuclear localization domain, a S-rich motif and a ACT domain. The constitutive expression of CdICE1 in C. grandiflorum improved the tolerance of C. grandiflorum to low temperature/freezing, drought and salinity. When the transgene was inserted in the antisense direction, the expression of the endogenous ICE1 gene was down-regulated, and the level of the plant's sensitivity to abiotic stress increased. The level of expression of CgDREBa and CgDREBb, activities of superoxide dismutase and peroxidase and the proline content were enhanced in the sense transgenic lines, and lowered in the antisense ones under stresses. In conclusion, CdICE1 represents a promising candidate for a biotechnological approach to improve the level of crop abiotic stress tolerance.
机译：各种非生物胁迫严重损害了菊花的质量和生产力。在这里，我们描述了使用RACE PCR从菊花中分离CdICE1的方法，该方法共享了来自菊花“ Jinba”品种的ICE1 ORF的相同核苷酸。 CdICE1包含一个保守的bHLH域，一个核定位域，一个富含S的基序和一个ACT域。 CdICE1在大花茶中的组成型表达提高了大花茶对低温/冷冻，干旱和盐分的耐受性。当在反义方向上插入转基因时，内源性ICE1基因的表达被下调，植物对非生物胁迫的敏感性水平也增加。在有义转基因品系中，CgDREBa和CgDREBb的表达水平，超氧化物歧化酶和过氧化物酶的活性以及脯氨酸含量在有压力的转基因品系中提高，而在反义品系中降低。总之，CdICE1代表了一种有望改善作物非生物胁迫耐受性的生物技术方法的有希望的候选人。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号