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High-affinity K+ transporter PhaHAK5 is expressed only in salt-sensitive reed plants and shows Na+ permeability under NaCl stress

机译:高亲和力K +转运蛋白PhaHAK5仅在盐敏感的芦苇植物中表达,并在NaCl胁迫下显示Na +渗透性

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摘要

To understand the mechanism of ion homeostasis in salt tolerant and sensitive plants, we isolated cDNAs for K(+) transporter PhaHAK1-u and PhaHAK5-u from reed plants. PhaHAK1-u belongs to group I and PhaHAK5-u belongs to group IV by phylogenetic analysis, respectively. PhaHAK5-u is predicted to be a plasma membrane transporter, and shows high-affinity K(+) transporter. Expression of PhaHAK5 was found in salt-sensitive reed plants, but not in any parts of salt-tolerant reed plants maintained under both control and K(+) starvation conditions. Under the NaCl stress, the K(+) uptake ability of the yeast strain expressing PhaHAK5-u was remarkably lower than that of the yeast strain expressing PhaHAK1-u, and PhaHAK5-u showed Na(+) permeability. These results suggest that PhaHAK5 is one of the routes by which Na(+) enters cells.
机译:为了了解耐盐和敏感植物中离子稳态的机制,我们从芦苇植物中分离了K(+)转运蛋白PhaHAK1-u和PhaHAK5-u的cDNA。通过系统进化分析,PhaHAK1-u属于I组,PhaHAK5-u属于IV组。预测PhaHAK5-u是质膜转运蛋白,并显示出高亲和力K(+)转运蛋白。在盐敏感的芦苇植物中发现了PhaHAK5的表达,但在对照和K(+)饥饿条件下维持的耐盐的芦苇植物的任何部分中均未发现。在NaCl胁迫下,表达PhaHAK5-u的酵母菌株的K(+)吸收能力显着低于表达PhaHAK1-u的酵母菌株,并且PhaHAK5-u显示Na(+)渗透性。这些结果表明,PhaHAK5是Na(+)进入细胞的途径之一。

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