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首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >Effect of MgCl2 and double concentration of Murashige and Skoog medium on in vitro plantlet and root cultures generation in halophytic grasswort Salicornia brachiata
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Effect of MgCl2 and double concentration of Murashige and Skoog medium on in vitro plantlet and root cultures generation in halophytic grasswort Salicornia brachiata

机译：MgCl2和Murashige和Skoog培养基的双重浓度对盐生苦草苦柳试管苗和根系生成的影响。

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An improved micropropagation protocol has been developed for halophytic grasswort Salicornia brachiata by using double concentration of Murashige and Skoog (Physiol Plant 15:473-497, 1962) medium (DMS) supplemented with plant growth regulators. Best shoot bud induction (90 %) from nodal explants was observed on DMS supplemented with 3.0 mg L-1 6-benzyl aminopurine (BAP) and 0.5 mg L-1 Zeatin with > 3 shoot buds. Shoot proliferation and elongation was achieved on DMS supplemented with 1.0 mg L-1 thidiazuron (TDZ) and 1.0 mg L-1 alpha-naphthalene acetic acid (NAA) with multiple shoot buds after 30 days of culture. Best callus response (78 %) was observed when stem explants were cultured on DMS supplemented with 2.0 mg L-1 2, 4-dichlorophenoxyacetic acid and 0.01 mg L-1 BAP. Regeneration from the callus was achieved when callus was cultured on DMS supplemented with 0.5 mg L-1 TDZ. These shoot buds were elongated on shoot proliferation and elongation medium. Elongated shoots (5 cm) could be rooted on DMS supplemented with 0.5-1.5 mg L-1 NAA, 0.5-1.5 mg L-1 indole-3-acetic acid and 0.5-1.5 mg L-1 indole-3-butyric acid. DMS medium supplemented with 0.5 mg L-1 NAA found to be best for rooting and the addition of 20 g L-1 magnesium chloride (MgCl2) to the medium resulted in highest percentage of rooting and not with the addition of sodium chloride (NaCl). Rooted plants could be established in soil with 55 % survival. In another set of study on root culture generation, many roots initiated from explants cultured on DMS supplemented with 2.0 mg L-1 NAA. Addition of 10 g L-1 MgCl2 was highly beneficial in stimulating root initiation and proliferation as compared to NaCl.
机译：通过使用补充有植物生长调节剂的Murashige和Skoog（Physiol Plant 15：473-497，1962）培养基的双倍浓度，已经为盐生的草梅草拟南芥开发了一种改良的微繁殖方案。在添加了3.0 mg L-1 6-苄基氨基嘌呤（BAP）和0.5 mg L-1 Zeatin并带有> 3个芽的DMS上，观察到来自节点外植体的最佳芽萌芽诱导（90％）。培养30天后，在补充有1.0 mg L-1噻唑隆（TDZ）和1.0 mg L-1α-萘乙酸（NAA）的DMS上实现了芽的增殖和伸长。当在添加了2.0 mg L-1 2、2，4-二氯苯氧基乙酸和0.01 mg L-1 BAP的DMS上培养茎外植体时，观察到最佳的愈伤组织反应（78％）。当在补充有0.5 mg L-1 TDZ的DMS上培养愈伤组织时，可以实现从愈伤组织的再生。这些芽在芽增殖和伸长培养基上伸长。伸长的芽（5厘米）可以扎在补充有0.5-1.5 mg L-1 NAA，0.5-1.5 mg L-1吲哚-3-乙酸和0.5-1.5 mg L-1吲哚-3-丁酸的DMS上。发现添加0.5 mg L-1 NAA的DMS培养基最适合生根，向培养基中添加20 g L-1氯化镁（MgCl2）导致生根的百分比最高，而不添加氯化钠（NaCl） 。可以在土壤中建立有根的植物，其存活率为55％。在另一组有关根培养物生成的研究中，许多根是由在补充了2.0 mg L-1 NAA的DMS上培养的外植体引发的。与NaCl相比，添加10 g L-1 MgCl2在刺激根部萌芽和增殖方面非常有益。

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