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首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >Ectopic expression of the apple Md-miR172e gene alters flowering time and floral organ identity in Arabidopsis
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Ectopic expression of the apple Md-miR172e gene alters flowering time and floral organ identity in Arabidopsis

机译：苹果Md-miR172e基因的异位表达改变拟南芥的开花时间和花器官身份

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摘要

The miRNA miR172 is involved in the regulation of flowering time and floral organ development by specifically restricting the transcripts of target gene APETELA2 (AP2) transcription factors. In our study, Md-miR172e and its target genes, MdAP2 and MdAP2-1 to MdAP2-7, were isolated from the apple cultivar Royal Gala (Malus x domestica). Phylogenetic tree analysis revealed that eight MdAP2 genes were similar to the AtAP2 subfamily and were putative targets of miR172. qRT-PCR and western blotting analyses indicated Md-miR172e regulation of the expression of the target gene MdAP2 at the translation level. Next, an over-expression construct 35S::Md-miR172e was generated and transformed into Arabidopsis. qRT-PCR showed that Md-miR72e expression and mature miR172e accumulation increased, and transgenic plants exhibited early flowering (20-30 days early in flowering) under long days and floral defects compared with wild-type. Taken together, these results suggest that miR172 and its target AP2-like genes are involved in flower developmental processes, particularly with regards to flowering time and floral organ development, and miR172 mediates a conserved regulatory pathway in apple and Arabidopsis.
机译：miRNA miR172通过特异性限制靶基因APETELA2（AP2）转录因子的转录本，参与开花时间和花器官发育的调控。在我们的研究中，Md-miR172e及其靶基因MdAP2和MdAP2-1至MdAP2-7从苹果品种Royal Gala（Malus x domestica）中分离出来。系统进化树分析显示，八个MdAP2基因与AtAP2亚家族相似，并且是miR172的假定靶标。 qRT-PCR和蛋白质印迹分析表明，Md-miR172e在翻译水平上调控靶基因MdAP2的表达。接下来，产生了过表达构建体35S :: Md-miR172e，并将其转化为拟南芥。 qRT-PCR显示，Md-miR72e的表达和成熟的miR172e的积累增加，转基因植物与野生型相比在较长的天数下表现出早期开花（开花早期20-30天）和花缺陷。综上所述，这些结果表明miR172及其靶标AP2类基因参与了花的发育过程，特别是在开花时间和花器官发育方面，并且miR172介导了苹果和拟南芥中保守的调控途径。

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