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首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >In vitro morphogenic responses of African yam bean (Sphenostylis stenocarpa (Hochst ex. A. Rich.) Harms) accessions to plant growth regulators
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In vitro morphogenic responses of African yam bean (Sphenostylis stenocarpa (Hochst ex. A. Rich.) Harms) accessions to plant growth regulators

机译：非洲山药豆（Sphenostylis stenocarpa（Hochst ex。A. Rich。）危害）对植物生长调节剂的体外形态发生反应

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摘要

Morphogenic responses of two accessions of African yam bean to different concentrations of plant growth regulator supplements to Murashige and Skoog basal medium was investigated to develop a more efficient regeneration system. Mature embryo explants were cultured on growth regulator-free and BAP + NAA supplemented media. Nodal cuttings excised from 4-week old shoots of the regenerated embryos were cultured on media containing varying concentrations and combinations of 6-benzyl aminopurine (BAP), kinetin and alpha-naphthalene acetic acid (NAA). Growth regulator-free medium favored embryo regeneration and growth over supplemented media and both enhanced shoot regeneration and rooting, but could not induce multiple shoot formation on embryo explants. Multiple shoots were produced by nodal explants and the highest average number of shoots (5.3 +/- 2.3), leaves (7.7 +/- 3.6), roots (3.7 +/- 2.9) and root length (3.1 +/- 0.0 cm) were obtained on a medium with 0.6 mg l(-1) BAP + 0.03 mg l(-1) NAA for accession TSs154, while in TSs5, highest number of shoots (3.2 +/- 2.5) and leaves (5.9 +/- 1.5) were induced by 2.0 mg l(-1) Kinetin + 0.05 mg l(-1) NAA. Such differential morphogenic responses to culture media underline the genotypic control of in vitro propagation of this crop. Embryo and nodal explants rooted directly on shoot regeneration media, and regenerated plantlets were successfully acclimatized. The efficient regeneration system obtained will enhance genetic improvement of African yam bean by facilitating molecular genetic transformation for advanced breeding.
机译：研究了两种山药豆对不同浓度的植物生长调节剂补充品对Murashige和Skoog基础培养基的形态发生反应，以开发更有效的再生系统。成熟的胚胎外植体在无生长调节剂和补充BAP + NAA的培养基上培养。从再生胚胎的4周龄幼芽切下的节状插条在含有不同浓度和6-苄基氨基嘌呤（BAP），激动素和α-萘乙酸（NAA）组合的培养基上培养。不含生长调节剂的培养基比补充培养基更有利于胚芽的再生和生长，并能增强枝条的再生和生根，但不能诱导胚芽在外植体上形成多芽。节点外植体可产生多芽，最高平均芽数（5.3 +/- 2.3），叶（7.7 +/- 3.6），根（3.7 +/- 2.9）和根长（3.1 +/- 0.0 cm）在含有0.6 mg l（-1）BAP + 0.03 mg l（-1）NAA的培养基上获得TSs154，而在TSs5中，芽的最高数量（3.2 +/- 2.5）和叶片（5.9 +/- 1.5） ）由2.0 mg l（-1）激动素+ 0.05 mg l（-1）NAA诱导。对培养基的这种不同的形态发生反应强调了该作物体外繁殖的基因型控制。胚和节点外植体直接植根于嫩芽再生培养基上，并且再生的幼苗已成功地适应环境。所获得的有效再生系统将通过促进先进遗传育种的分子遗传转化来增强非洲山药豆的遗传改良。

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