• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra
【24h】

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

机译：北美重要栎树栎，双色栎和红栎的离体再生

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

North American oak species, with their characteristic strong episodic seasonal shoot growth, are highly problematic for clonal micropropagation, resulting in the inability to achieve a stabilized shoot multiplication stage. The potential for initiating and proliferating shoot cultures derived from Quercus alba, Q. bicolor and Q. rubra explants was investigated, and a micropropagation method for these species was developed. Branch segments from 6 to 7-year-old trees were forced-flushed and the forced shoots were used as source of explants for culture initiation. A consistent shoot multiplication stage was achieved, in 13 of the 15 genotypes established in vitro, although marked differences occurred in explants from different genotypes/species. The control of efficient shoot multiplication involved the culture of decapitated shoots in a stressful horizontal position on cytokinin-containing medium with a sequence of transfers within a 6-week subculture cycle, which was beneficial to overcoming the episodic character of shoot growth. During each subculture cycle, the horizontally placed explants were cultured on media containing 0.2 mg lp# benzyladenine (BA) for 2 weeks with two successive transfers (2 weeks each) to fresh medium with 0.1 mg lp# BA, giving a 6-week subculture cycle. The general appearance and vigor of Q. alba and Q. bicolor shoot cultures were improved by the inclusion of both 0.1 mg lp# BA and 0.5 mg lp# zeatin in the medium used for the second transfer within the 6-week subculture cycle. Addition of AgNO (3 mg lp#) to the shoot proliferation medium of Q. rubra had a significant positive effect on shoot development pattern by reducing deleterious symptoms, including shoot tip necrosis and early senescence of leaves. The three species showed acceptable in vitro rooting rates by culturing microcuttings in medium containing 25 mg lp# indolebutyric acid for 48 h with subsequent transfer to auxin-free medium supplemented with 0.4% activated charcoal. Although an initial 5-day dark period generally improved the rooting response, it was detrimental to the quality of regenerated plantlets. However, activated charcoal stimulated not only the rooting frequencies, but it also enhanced plant quality, as evidenced by root, shoot and leaf growth.
机译：北美橡树种，其特征性的强壮的季节季节性芽生长，对于克隆微繁来说存在很大的问题，导致无法实现稳定的芽繁殖阶段。研究了启动和增殖来自白栎，双色和Q. rubra外植体的芽培养物的潜力，并开发了用于这些物种的微繁殖方法。将6至7岁树的分支部分强行清除，并将强制芽用作培养起始的外植体来源。在体外建立的15个基因型中，有13个达到了一致的芽繁殖阶段，尽管来自不同基因型/物种的外植体发生了显着差异。有效芽繁殖的控制涉及在含有细胞分裂素的培养基上以压力水平位置对断头的芽进行培养，并在6周的继代培养周期内进行一系列转移，这有利于克服芽生长的间歇性特征。在每个亚培养周期中，将水平放置的外植体在含有0.2 mg lp＃苄基腺嘌呤（BA）的培养基上培养2周，然后连续两次转移（每次2周）到含有0.1 mg lp＃BA的新鲜培养基中，进行6周的亚培养周期。通过在6周的继代培养周期内第二次转移所用的培养基中同时加入0.1 mg lp＃BA和0.5 mg lp＃玉米素，可以改善Q. alba和Q. bicolor芽培养的总体外观和活力。向白菜Q的枝条增殖培养基中添加AgNO（3 mg lp＃）通过减少有害症状，包括枝梢坏死和叶片早衰，对枝条发育模式具有显着的正作用。通过在包含25 mg lp＃吲哚丁酸的培养基中培养微切屑48小时，随后转移到补充有0.4％活性炭的无生长素的培养基中，这三个物种显示出可接受的体外生根率。尽管最初的5天黑暗期通常可以改善生根响应，但它对再生苗的质量有害。但是，活性炭不仅能刺激生根频率，而且还能提高植物的品质，这可以从根，茎和叶的生长情况看出。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号