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首页> 外文期刊>Plant Disease >First report of Lasidiplodia theobromae and Cryptovalsa ampelina associated with grapevine decline from Castilla y Leon, Spain.
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First report of Lasidiplodia theobromae and Cryptovalsa ampelina associated with grapevine decline from Castilla y Leon, Spain.

机译：西班牙卡斯蒂利亚-莱昂的葡萄藤衰弱有关的 Lasidiplodia theobromae 和 Cryptovalsa ampelina 的首次报道。

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Symptoms of grapevine decline were surveyed. Samples from mature vines exhibiting external symptoms of Eutypa dieback and Esca were collected, as were young plants with and without external symptoms, and fungal isolations were performed. In 2007, three-year-old grapevines (cv. Trempranillo grafted onto 110R rootstock) with low vigour, reduced foliage and vascular streaking in the wood were observed. Lasiodiplodia theobromae [Botryodiplodia theobromae] was identified based on morphological characteristics and confirmed by banding patterns obtained after the digestion of the 1200 bp amplicon generated with ITS 1 and NL4 primers using restriction endonucleases. L. theobromae appeared to be a more aggressive pathogen than Cryptovalsa ampelina on grapevine with more internal brown streaking and greater recovery of pathogen from inoculated samples. This is thought to be the first report of L. theobromae and C. ampelina causing grapevine decline in Castilla y Leon.
机译：调查了葡萄下降的症状。收集来自表现出Eutypa死亡和Esca外部症状的成熟葡萄的样品，以及有或没有外部症状的年轻植物，并进行真菌分离。在2007年，观察到了三岁的葡萄藤（葡萄树Trempranillo嫁接到110R砧木上），活力低，树木的叶子减少和血管斑纹。基于形态特征鉴定了 Lasiodiplodia theobromae [Botryodiplodia theobromae ]，并通过使用限制性核酸内切酶消化了由ITS 1和NL4引物消化的1200 bp扩增子后获得的条带图谱来证实。 L。在葡萄树上，theobromae 似乎是一种比 Cryptovalsa ampelina 更具侵略性的病原体，内部有更多的棕色条纹，并且从接种样品中回收的病原体更多。这被认为是 L的第一份报告。 ob科和 C。 ampelina 导致卡斯蒂利亚和莱昂葡萄的衰落。

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