首页> 外文期刊>Plant Disease >First report of bot canker caused by Diplodia corticola on coast live oak (Quercus agrifolia) in California.
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First report of bot canker caused by Diplodia corticola on coast live oak (Quercus agrifolia) in California.

机译:由加利福尼亚州的沿海橡树( Quercus agrifolia )的Diplodia corticola 引起的僵尸病首次报道。

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Sharp decline and mortality of coast live oak (Q. agrifolia) has been observed in San Diego County, California, USA, since 2002. Much of this decline has been attributed to a new pest in California, the goldspotted oak borer (GSOB; Agrilus coxalis). Symptoms include crown thinning, bark cracking and/or peeling, patches of stain (1-10 cm in diameter), bleeding on the bole, and tree death, and are most often observed on trees with a diameter at breast height of more than 30 cm. In 2008, a Botryosphaeria sp. was recovered from necrotic tissue of bleeding bole cankers from GSOB-affected trees in Jamul, California. Zone lines separated dead and live tissue in affected phloem and xylem. Pycnidia were observed on the bark surface of the infected host. Oak stands with tree mortality were surveyed in GSOB-infested and -uninfested sites over eight locations throughout San Diego and Riverside counties in 2009 and 2010. Symptomatic tissue or conidia from pycnidia of affected trees, plated onto potato dextrose agar amended with 0.01% tetracycline and incubated at 25 degrees C for one week, consistently produced cultures with dense, wooly, olive-green mycelium. Mycelia fit the description of B. corticola (anamorph= D. corticola). The resulting amplified ITS4/5 region of two sequences matched 100% to published D. corticola sequences (GU799472 and GU799460). This is thought to be the first report of D. corticola causing bot canker on coast live oak in California.
机译:自2002年以来,在美国加利福尼亚州的圣地亚哥县,沿海活动橡树( Q。agrifolia )的数量急剧下降,死亡率下降。这种下降的主要原因是加利福尼亚的一种新病虫害。金斑橡木bore(GSOB; Agrilus coxalis )。症状包括树冠变薄,树皮开裂和/或剥落,斑块(直径为1-10厘米),胆汁出血和树木死亡,并且最常见于胸径大于30的树木厘米。在2008年, Botryosphaeria sp。从加利福尼亚贾穆尔受GSOB影响的树木的出血性溃疡病的坏死组织中回收。区域线将受影响的韧皮部和木质部中的死组织和活组织分开。在受感染宿主的树皮表面观察到了股癣。在2009年和2010年期间,在圣地亚哥和河沿县的8个地方的GSOB感染和未感染的地点调查了具有树木死亡率的橡树林。将患病树的孢子虫的症状组织或分生孢子铺在马铃薯葡萄糖琼脂上,用0.01%四环素和在25摄氏度下孵育1周,始终产生带有致密,羊毛状,橄榄绿色菌丝体的培养物。菌丝体符合 B的描述。 corticola (anamorph = D。corticola )。所得的两个序列的扩增的ITS4 / 5区域与公开的D匹配100%。皮质序列(GU799472和GU799460)。这被认为是 D的第一份报告。皮质醇在加利福尼亚州的沿海橡树上引起了僵尸病。

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