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首页> 外文期刊>Plant Disease >Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes
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Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes

机译：基于Ⅲ型效应蛋白基因的巴西柑桔黄单胞菌菌株的遗传多样性

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Jaciani, F. J., Ferro, J. A., Ferro, M. I. T., Verniere, C., Pruvost, O., and Belasque, J., Jr. 2012. Genetic diversity of a Brazilian strain collection of Xanthomonas citri subsp. citri based on the type III effector protein genes. Plant Dis. 96:193-203. Exclusion and eradication or management based on an integrated approach with less susceptible varieties, copper-based bactericides, and windbreaks are the two main strategies used to prevent or control citrus canker. Field tolerance or resistance to citrus canker is not found in the most important commercial sweet orange cultivars, and pathogen-derived resistance has been developed and applied in different crops to obtain resistant genotypes to plant pathogens. We describe the development of DNA primers and probes based on the type III effector genes avrXacE1, avrXacE2, avrXacE3, avrBs2, pthA4, hpaF, and XAC3090 (leucine rich protein), and their application in the evaluation of the genetic diversity of the pathogen. A total of 49 haplotypes were identified in 157 strains by Southern blot analysis. No genetic polymorphism was detected by BOX elements - and enterobacterial repetitive inter-genic consensus polymerase chain reaction (ERIC-PCR) analysis, nor with the genes avrBs2, XAC3090, and hpaF. Nei's genetic diversity indexes varied from 0.65 to 0.96 for subcollections of the pathogen. One or few haplotypes were most frequent in the strain collection, but several haplotypes were represented by solely one or few strains. The PthA4 probe resulted in the higher number of haplotypes identified in the Brazilian subcollections. Greater variation in the frequency of haplotypes occurred within subcollections (93.7%) than among subcollections. Only some haplotypes were genetically distant from all others, especially those originated from Rio Grande do Sul and Santa Catarina states. These bacterial effectors are widely spread in the collections and are useful for a better understanding of the host pathogen interaction and the search for resistance genes in host and nonhost plants.
机译：Jaciani，F. J.，Ferro，J. A.，Ferro，M.I. T.，Verniere，C.，Pruvost，O.和Belasque，J.，Jr.2012。巴西黄单胞菌Xanthomonas citri subsp。 citri基于III型效应蛋白基因。植物病96：193-203。基于易感品种，铜基杀菌剂和防风林的综合方法进行的排斥和根除或管理，是预防或控制柑橘溃疡病的两种主要策略。在最重要的商业甜橙品种中未发现对柑橘溃疡病的田间耐受性或抗性，并且已经开发出病原体衍生的抗性并将其应用于不同作物中以获得对植物病原体的抗性基因型。我们描述了基于III型效应基因avrXacE1，avrXacE2，avrXacE3，avrBs2，pthA4，hpaF和XAC3090（富含亮氨酸的蛋白）的DNA引物和探针的开发，以及它们在病原体遗传多样性评估中的应用。通过Southern印迹分析在157个菌株中鉴定出总共49个单倍型。 BOX元件和肠细菌重复性基因间共有聚合酶链反应（ERIC-PCR）分析，以及avrBs2，XAC3090和hpaF基因均未检测到遗传多态性。 Nei的病原体亚种的遗传多样性指数从0.65到0.96不等。菌株收集中最常见一种或几种单倍型，但仅一种或几种菌株代表几种单倍型。 PthA4探针导致在巴西子集合中鉴定出更高数量的单倍型。与子集合相比，子集合中单倍型频率发生的变化更大（93.7％）。只有一些单倍型在遗传上与其他所有单倍型相距遥远，尤其是那些起源于南里奥格兰德州和圣卡塔琳娜州的单倍型。这些细菌效应子广泛分布在集合中，可用于更好地了解宿主病原体的相互作用以及在宿主和非宿主植物中寻找抗性基因。

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