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首页> 外文期刊>Plant Disease >Seasonal Susceptibility of Grapevine Pruning Wounds and Cane Colonization in Catalonia, Spain Following Artificial Infection with Diplodia seriata and Phaeomoniella chlamydospora
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Seasonal Susceptibility of Grapevine Pruning Wounds and Cane Colonization in Catalonia, Spain Following Artificial Infection with Diplodia seriata and Phaeomoniella chlamydospora

机译：人工感染梁龙和衣原体衣原体后，西班牙加泰罗尼亚的葡萄修剪伤口和甘蔗定植的季节性敏感性

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摘要

Diplodia seriata and Phaeomoniella chlamydospora are two fungal pathogens associated with grapevine trunk diseases worldwide. This study aimed to evaluate the period during which grapevine pruning wounds remain susceptible to fungal infection and to describe the colonization of canes artificially inoculated with these pathogens. In the first experiment, pruning wounds made in either fall or winter were separately inoculated with each pathogen at different times after pruning. Wound susceptibility to both pathogens decreased as the period between pruning and inoculation increased, from high percentages recorded in the first inoculation round (D. seriata, 97.5% and P. chlamydospora, 75%) down to approximately 10% 12 weeks after pruning. Pruning wounds remained more susceptible to D. seriata after a late pruning in winter whereas no overall seasonal changes in wound susceptibility were detected for P. chlamydospora. In the second experiment, canes were pruned by leaving two different lengths between the top node and the pruning wound before inoculations. Pathogens were recovered at different incubation periods and from different sites along the canes to estimate fungal cane colonization. A longer pruned internode made cane colonization by P. chlamydospora difficult, as indicated by fungal recoveries lower than 10% at the lowest recovery site, whereas D. seriata was less inhibited.
机译：Diplodia seriata和Phaeomoniella chlamydospora是与全世界葡萄干病相关的两种真菌病原体。这项研究的目的是评估葡萄树修剪伤口对真菌感染的敏感性，并描述人工接种这些病原体的甘蔗定植时间。在第一个实验中，在修剪后的不同时间分别为每种病原体接种秋季或冬季修剪的伤口。修剪和接种之间的时间间隔越长，对两种病原体的伤口敏感性就越低，从第一轮接种中的高百分比（D. seriata，97.5％和P. chlamydospora，75％）下降到修剪后12周的约10％。冬季后期修剪后，修剪的伤口仍更容易受到D. seriata的侵害，而对于衣原体P. chlamydospora，未发现伤口敏感性的总体季节性变化。在第二个实验中，通过在接种前在顶节和修剪伤口之间留出两个不同的长度来修剪藤茎。在不同的潜伏期和沿甘蔗的不同部位回收病原体，以估计真菌甘蔗定植。较长的修剪节间使藤茎假单胞菌难以定植，这是由最低回收位点的真菌回收率低于10％所表明的，而对D. seriata的抑制则较少。

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