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Characterization of Rhizoctonia solani Associated with Black Scurf in Cyprus

机译：在塞浦路斯的黑衣草伴有solani Rhizoctonia solani的表征

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During 2011, 96 sclerotial isolates of Rhizoctonia solani were collected from potato tubers from all main potato-cultivating regions of Cyprus. All isolates were found to be multinucleate. Characterization of anastomosis groups (AG) based on hyphal anastomosis reactions showed that 91 isolates belonged to AG3 and 5 to AG4. Sequence analysis of the internal transcribed spacer (ITS) regions (ITS1 and ITS2) of ribosomal DNA (rDNA) of 68 isolates confirmed the prevalence of AG3. In addition, phylogeneticanalysis found that AG3 isolates were of the potato type, distinctly separated from the AG3 tobacco type, while AG4 isolates were separated into two different subgroups (HGI and HGII). Temperature studies showed that isolates belonging to both AG4 subgroups had significantly higher optimum growth temperatures compared with AG3. In vitro sensitivities to the fungicide pencycuron, in terms of concentrations where 50% growth inhibition was observed, ranged from0.012 to 0.222 p-g/ml. Pathogenicity and aggressiveness of the isolates was determined on 'Annabelle' potato sprouts and seedlings of a number of selected hosts, based on crop rotations followed in Cyprus. The majority of the isolates were pathogenic topotato sprouts, with disease severity (DS) values ranging from 0 to 88%. Mean DS values were statistically different among AG and subgroups, with AG4-HGI (69.25%) and AG4-HGII (3.12%) being the most and least aggressive, respectively. However, AG4-HGII isolates were the most aggressive in all rotational hosts tested, while AG3 isolates were the least aggressive. More specifically, the highest DS levels by AG4-HGI were recorded to barley, by AG4-HGII to lettuce and melon, and by AG3 isolates to vetch. This is the first comprehensive study to elucidate the AG composition, pathogenicity and other biological aspects of R. solani isolates associated with potato black scurf in Cyprus.
机译：2011年期间，从塞浦路斯所有主要马铃薯种植区的马铃薯块茎中收集了96株立枯丝核菌菌核分离株。发现所有分离物都是多核的。基于菌丝吻合反应的吻合组（AG）的特征表明91个分离株属于AG3，5个属于AG4。 68个分离株的核糖体DNA（rDNA）的内部转录间隔区（ITS）区（ITS1和ITS2）的序列分析证实了AG3的流行。此外，系统发育分析发现，AG3分离株属于马铃薯类型，与AG3烟草类型明显分开，而AG4分离株则分为两个不同的亚组（HGI和HGII）。温度研究表明，与AG3相比，属于这两个AG4亚组的分离株的最佳生长温度明显更高。就观察到50％生长抑制的浓度而言，对杀真菌剂蓬库仑的体外敏感性范围为0.012至0.222 p-g / ml。根据塞浦路斯随后的轮作，在许多选定寄主的“ Annabelle”马铃薯芽和幼苗上确定了分离物的致病性和侵袭性。大部分分离物是致病性马铃薯芽，病害严重度（DS）值范围为0至88％。 AG和亚组之间的平均DS值在统计学上不同，AG4-HGI（69.25％）和AG4-HGII（3.12％）分别是最具攻击性和最小攻击性。但是，在所有测试的旋转宿主中，AG4-HGII分离株的攻击性最高，而AG3分离株的攻击性最低。更具体地说，通过AG4-HGI记录的最高DS含量记录在大麦中，通过AG4-HGII记录在生菜和甜瓜中，通过AG3分离株记录在紫etch中。这是第一项在塞浦路斯阐明与马铃薯黑皮屑相关的茄红枯菌的AG组成，致病性和其他生物学方面的综合研究。

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