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Overexpression of OsOSM1 Enhances Resistance to Rice Sheath Blight

机译：OsOSM1的过表达增强了对水稻纹枯病的抵抗力

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摘要

Sheath blight (SB), caused by Rhizoctonia solani, is one of the most destructive rice diseases worldwide. It has been difficult to generate SB-resistant varieties through conventional breeding because of the quantitative nature of rice resistance to SB. In this study, we found that overexpression of the OsOSM1 gene, encoding an osmotin protein belonging to the pathogenesis-related protein 5 family, is able to improve rice resistance to SB in field tests. Although there are two osmotin genes in rice, OsOSM1 is the one mainly expressed in leaf sheath at the booting stage, coinciding with the critical stage of SB development in the field. In addition, OsOSM1 expression is strongly induced by R. solani in SB-resistant rice variety YSBR1 but not in susceptible varieties, suggesting its involvement in SB resistance. Overexpression of OsOSM1 (OsOSM1ox) in susceptible variety Xudao 3 significantly increases resistance to SB in transgenic rice. The OsOSM1 mRNA levels in different transgenic lines are found to be positively correlated with their SB resistance levels. Intriguingly, although extremely high levels of OsOSM1 were detrimental to rice development, appropriately elevated levels of OsSOM1 were obtained that enhanced rice SB resistance without affecting rice development or grain yield. The OsSOM1 protein is localized on plasma membrane. OsOSM1 is upregulated by jasmonic acid (JA); furthermore, JA-responsive marker genes are induced in OsOSM1ox lines. These results suggest that the activation of JA signaling pathway may account for the increased resistance in transgenic OsOSM1ox lines. Taken together, our results demonstrate that OsOSM1 plays an important role in defense against rice SB disease and provides a new target for engineering resistance to SB.
机译：茄枯萎病（SB）是由Rhizoctonia solani引起的，是世界上最具破坏性的水稻疾病之一。由于水稻对SB的抗性具有定量性，因此难以通过常规育种产生抗SB的品种。在这项研究中，我们发现，OsOSM1基因的过表达（编码属于发病相关蛋白5家族的渗透蛋白）能够在田间试验中提高水稻对SB的抗性。尽管水稻中有两个渗透素基因，但OsOSM1是在启动阶段主要在叶鞘中表达的一个基因，与该领域SB发育的关键时期相吻合。此外，OsOSM1的表达在抗SB水稻品种YSBR1中被sol。R. solani强烈诱导，但在易感品种中没有表达，表明它参与了SB抗性。在易感品种徐道3号中过表达OsOSM1（OsOSM1ox）显着提高了转基因水稻对SB的抗性。发现不同转基因品系中的OsOSM1 mRNA水平与其SB抗性水平呈正相关。有趣的是，尽管极高水平的OsOSM1不利于水稻的发育，但获得了适当升高的OsSOM1水平，可增强水稻对SB的抗性而不影响水稻的发育或籽粒产量。 OsSOM1蛋白位于质膜上。茉莉酸（JA）会上调OsOSM1;此外，在OsOSM1ox系中诱导了JA反应性标记基因。这些结果表明，JA信号通路的激活可能解释了转基因OsOSM1ox品系的抗性增加。综上所述，我们的结果表明，OsOSM1在防御水稻SB病中起着重要作用，并为工程抗SB提供了新的目标。

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